来自真菌和昆虫病原菌Moelleriella的线粒体基因组属揭示了进化历史,内部动力学和原生学
Chengjie Xiong1, Yongsheng Lin1, Nemat O Keyhani2
1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Journal of fungi (Basel, Switzerland)
|February 25, 2025
概括
这项研究介绍了七种*Moelleriella*昆虫病原体的第一个线粒体基因组. 内部变异显著影响真菌基因组大小和进化,揭示了与*Metarhizium*的密切关系.
科学领域:
- 菌类学 菌类学是指菌类学.
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- *Moelleriella*真菌是特定于虫和白的昆虫病原体.
- 以前,对*Moelleriella*物种*的线粒体基因组无法获得,这限制了基因组和进化研究.
研究的目的:
- 组装和分析来自*Moelleriella*属的前七个线粒体基因组.
- 为了研究基因组大小变异,基因含量,密码子的使用,以及Moelleriella*内的进化压力.
- 为了确定Moelleriella在Hypocreales序列中的遗传位置.
主要方法:
- 测序和组装七个 *Moelleriella* 线粒基因组.
- 对合成,基因顺序和代码子使用的比较基因组分析.
- 分析蛋白质编码基因中的内部多态和序列变异.
- 使用核心蛋白质编码基因进行遗传学分析.
主要成果:
- 七个*Moelleriella*的线粒体从40.8到95.7Kb不等,具有显著的内部多态性,有助于大小变化.
- 在Hypocreales中保留了基因排列,并观察到净化选择压力 (ka/ks < 1).
- *atp6*基因显示最大的遗传距离,而*atp9*显示最小的遗传距离.
- 遗传学分析将七种*Moelleriella*物种置于一个得到良好支持的分类,与*Metarhizium*密切相关.
结论:
- 在*Moelleriella*和Hypocreales中,线粒体基因组大小的变化主要是由内核驱动的.
- 这项研究为*Moelleriella*提供了基础的基因组数据,进一步了解了它们的分类学,进化和基因组学.
- 这些发现确立了Moelleriella在Clavicipitaceae家族中的遗传关系.
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