一个强大的基于PET的性希夫基分子探针用于识别Hg2+和Pb2+及其在活细胞成像中的应用
S Benita Jeba Silviya1, S Michlin Ruphina Maragatham2, S G Jebastin Andrews3
1Department of Physics, Pope's College, Sawyerpuram - 628 251, Thoothukudi District, Tamil Nadu, India.
Analytical methods : advancing methods and applications
|February 25, 2025
概括
一个新的光传感器,H2L,检测有毒重金属 (Hg2+) 和 (Pb2+) 的高灵敏度和选择性. 这种Schiff基底传感器显示了环境和生物应用的潜力.
科学领域:
- 化学传感器 化学传感器
- 材料科学 材料科学 材料科学
- 分析化学 分析化学
背景情况:
- 像 (Hg2+) 和 (Pb2+) 这样的重金属离子对环境和健康构成重大风险.
- 开发用于重金属检测的选择性和敏感传感器对于监测和补救至关重要.
研究的目的:
- 设计和合成一种新的基于盐酸的奇拉 Schiff 基 (H2L) 作为光传感器.
- 为了研究传感器检测Hg2+和Pb2+离子的能力.
- 探索传感器在现实世界样本和生物系统中的应用.
主要方法:
- 希夫基H2L的合成.
- 使用单晶X射线衍射进行表征.
- 光谱学用于研究离子结合和传感机制 (CHEF和PET效应).
- 使用乔布的图表,1H NMR定位和ESI-MS,验证绑定模式.
- 在水样,纸条和HeLa细胞中进行应用测试.
主要成果:
- 成功合成并确认H2L的结构.
- H2L具有选择性和敏感的光检测Hg2+和Pb2+.
- 实现了Hg2+的8.29nM和Pb2+的7.65nM的低检测极限 (LOD).
- 在真实水样,纸条和HeLa细胞中进行了有效的检测.
结论:
- 合成的H2L作为一种高效的光传感器,用于检测Hg2+和Pb2+.
- 传感器通过CHEF和PET机制工作,显示出高选择性和灵敏度.
- H2L证明了在各种环境和生物环境中监测重金属的实用性.
相关概念视频
Prochirality
3.8K
The concept of prochirality leads to the nomenclature of the individual faces of a molecule and plays a crucial role in the enantioselective reaction. It is a concept where two or more achiral molecules react to produce chiral products. A typical process is the reaction of an achiral ketone to generate a chiral alcohol. Here, the achiral reactant reacts with an achiral reducing agent, sodium borohydride, to generate an equimolar mixture of the chiral enantiomers of the product. For example, an...
3.8K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
618
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
618
Chirality at Nitrogen, Phosphorus, and Sulfur
5.7K
Chirality is most prevalent in carbon-based tetrahedral compounds, but this important facet of molecular symmetry extends to sp3-hybridized nitrogen, phosphorus and sulfur centers, including trivalent molecules with lone pairs. Here, the lone pair behaves as a functional group in addition to the other three substituents to form an analogous tetrahedral center that can be chiral.
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
5.7K
¹H NMR Chemical Shift Equivalence: Enantiotopic and Diastereotopic Protons
1.4K
Replacing each alpha-hydrogen in chloroethane by bromine (or a different functional group) yields a pair of enantiomers. Such protons are called prochiral or enantiotopic and are related by a mirror plane. Enantiotopic protons are chemically equivalent in an achiral environment. Because most proton NMR spectra are recorded using achiral solvents, enantiotopic hydrogens yield a single signal.
In chiral compounds such as 2-butanol, replacing the methylene hydrogens at C3 produces a pair of...
In chiral compounds such as 2-butanol, replacing the methylene hydrogens at C3 produces a pair of...
1.4K
Amines to Sulfonamides: The Hinsberg Test
3.2K
The Hinsberg test is a method to identify primary, secondary and tertiary amines, named after its pioneer, Oscar Hinsberg. Here, amines are treated with benzenesulfonyl chloride, also known as the Hinsberg reagent, in the presence of an excess of aqueous base, followed by acidification. Based on the nature of the amines, different changes are observed.
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing...
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing...
3.2K


