相关实验视频

Updated: May 1, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

在微粒物质诱导的肺损伤中对N6-甲基亚诺辛修饰的长非编码RNA进行全转录组分析

Yingying Zeng^1,2, Guiping Zhu^1,2, Wenjun Peng^1,2

¹Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200030, China.

|February 25, 2025

概括

这项研究揭示了与颗粒物 (PM) 诱导的肺损伤相关的长非编码RNA (lncRNAs) 中N6-甲基亚诺辛 (m⁶A) 修饰的新见解. 确定了三个关键的m⁶A-修饰的lncRNAs,揭示了肺病原发生的新调节机制.

科学领域:

分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
环境健康环境健康

背景情况:

N6-甲基氨酸 (m⁶A) 修饰调节受环境因素影响的细胞过程.
在颗粒物 (PM) 诱导的肺损伤中,m6A修饰的长非编码RNA (lncRNAs) 的作用在很大程度上是未知的.

研究的目的:

为了研究m⁶A-修饰的lncRNAs在PM诱导的肺损伤中的作用.
为了确定参与肺损伤病原发生的特定m⁶A-修饰的lncRNAs.

主要方法:

建立了PM诱导的肺损伤的小鼠模型.
使用m6A修饰RNA免疫沉测序 (MeRIP-seq) 和lncRNA测序 (lncRNA-seq) 来识别差异表达的m6A峰值和lncRNAs.
生物信息分析和qRT-PCR用于数据分析和验证.

主要成果:

在644个和358个lncRNA中分别发现了664个高甲基化和367个低甲基化m6A峰值.
生物信息分析表明,它与炎症,免疫反应和新陈代谢有关.
三个关键的m⁶A-修饰的lncRNAs (NR_003508,uc008scb.1,ENSMUST00000159072) 被确定并验证.

结论:

这项研究提供了第一个全面的转录组全方位分析m⁶与PM诱导的肺损伤相关的lncRNA中的甲基化概况.
确定了三个关键候选m⁶A-修饰的lncRNAs,这表明PM诱导的肺损伤中存在一种新的调节机制.

关键词:

长非编码RNAs的编码.肺部受伤的原因是肺部受伤.m6A甲基化的情况.颗粒物质颗粒物质颗粒物质

更多相关视频

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

相关概念视频

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...