打破障碍:甘地达利辛破坏了上皮质的完整性,并在肠上芯片模型中诱导炎症
Moran Morelli1, Karla Queiroz1
1MIMETAS B.V., De Limes 7, 2342 DH Oegstgeest, The Netherlands.
Toxins
|February 25, 2025
概括
虫 Candida albicans candidalysin 损害了肠道屏障,导致炎症和细胞损伤. 这项研究开发了gut-on-chip模型,以评估候选分析素.
科学领域:
- 微生物学 微生物学
- 病变的发生和发病.
- 毒理学 毒理学 毒理学
背景情况:
- 香虫是一种机会性病原体,引起全身感染.
- 香素是一种由C. albicans分泌的毒素,可以突破表皮屏障.
- 甘地利辛对表皮反应的确切影响需要进一步的表征.
研究的目的:
- 为了表征表皮细胞对 kandidatalysin 的反应.
- 开发可扩展的定量方法来评估候选素的毒理作用.
- 使用肠上芯片模型来研究候选分析因引起的损伤.
主要方法:
- 在OrganoPlate®平台上对Caco-2管道暴露于candylysin.
- 使用跨上皮电阻 (TEER) 评估屏障完整性.
- 通过蛋白质检测 (LDH,细胞因子,LL37) 和免疫染来评估细胞损伤和免疫反应.
主要成果:
- 香素损害了上皮屏障的完整性,并增加了细胞的透性.
- 观察到动蛋白重塑和细胞损伤.
- 释放的LDH,细胞因子和LL37表明了炎症和抗菌活性.
结论:
- 甘地达利辛通过破坏表皮屏障,在C. albicans的病变发生中发挥着重要作用.
- 肠上芯片模型提供了一个强大的平台来研究candidalysin的影响.
- 向candidalysin提供了针对C. albicans感染的潜在治疗策略.
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