碳源,细胞密度,以及微生物群落控制抑制V. 霍乱是环境酸盐的表面殖民
Jamaurie James1, Renato E R S Santos1,2, Paula I Watnick1,2
1Division of Infectious Diseases, Boston Children's Hospital, Boston, Massachusetts, USA.
mBio
|February 25, 2025
概括
霍乱病毒生物膜的形成被酸盐抑制,这是其酸盐呼吸的副产品. 像Paracoccus aminovorans这样的肠道微生物可以代谢这种化物,拯救生物膜的形成并增加V. cholerae的殖民.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 肠道微生物组研究研究
背景情况:
- 霍乱病毒在一个微空气友好的,缺乏葡萄糖的,富含酸盐的环境中殖民于终端结膜.
- 在葡萄糖稀缺期间,V. cholerae利用cAMP受体蛋白 (CRP) 来利用营养.
- 霍乱病毒的酸盐呼吸导致酸盐的积累,抑制生物膜的形成.
研究的目的:
- 调查CRP在V.霍乱菌酸盐呼吸中的作用.
- 为了阐明酸盐介导的抑制V. cholerae生物膜形成的机制.
- 确定肠道微生物是否可以减轻酸盐抑制并增强V. cholerae的殖民.
主要方法:
- 霍乱病毒的空气培养被用来研究酸盐呼吸.
- 与V. cholerae和Paracoccus aminovorans共同培养的实验是在酸盐补充的介质中进行的.
- 进行了对V. cholerae生物膜形成和表面积累的分析.
主要成果:
- 霍乱病毒酸盐呼吸是由CRP和葡萄糖可用性控制的.
- 霍乱病毒产生的化物抑制生物膜的形成,这取决于HapR调节器.
- 帕拉科库斯阿米诺瓦朗斯代谢V.霍乱产生的亚酸盐,增强V.霍乱表面积累.
结论:
- 结核中的V. cholerae生物膜的形成受到自身产生的酸盐的限制.
- 像P.aminovorans这样的肠道微生物可以通过消耗酸盐来拯救V.cholerae生物膜的形成.
- 肠道微生物群中酸盐代谢微生物的存在可能会影响V. cholerae的殖民化和霍乱易感性.
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