相关实验视频
Updated: May 26, 2025

13:00
Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
2.3K
在SARS-CoV-2的传染性可以通过细菌修饰糖核的调节
Cameron Martino1,2,3, Benjamin P Kellman1,2, Daniel R Sandoval4
1Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, California, USA.
mBio
|February 25, 2025
概括
肠道细菌在肠道中修改肝硫酸盐 (HS),影响严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 感染. 细菌酶减少病毒结合和感染,这表明它们在疾病易感性方面发挥了作用.
科学领域:
- 微生物组研究的研究.
- 病毒学 病毒学
- 胃肠病学 胃肠病学
背景情况:
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 感染了胃肠道 (GI),胃肠道症状与疾病严重程度相关.
- SARS-CoV-2 进入宿主细胞依赖于细胞表面和肠道粘膜中存在的肝酸硫酸 (HS) 蛋白质糖.
- 众所周知,食用肠道细菌会改变HS,可能会影响肠道内的病毒相互作用.
研究的目的:
- 为了研究人类肠道中HS修饰细菌的丰富性.
- 确定这些细菌种群如何与SARS-CoV-2感染的背景下与年龄,性别,疾病严重程度,并发症和饮食相关.
- 探索细菌HS修饰对SARS-CoV-2感染性的影响.
主要方法:
- 基于任务的蛋白质甘氨酸降解分析,应用于大规模的猎枪元基因组数据.
- 对肠道微生物组测序数据集的分析.
- 在体外实验中使用真实的SARS-CoV-2和工程化益生菌 (Escherichia coli Nissle 1917) 的实验.
主要成果:
- 肠道细菌的丰富度具有高HS代谢能力,随着年龄和性别显著变化,这些因素与2019年冠状病毒病 (COVID-19) 严重程度有关.
- 这些细菌形状也与感染期间和感染后的疾病严重程度直接相关.
- 根据COVID-19并发症或饮食,没有观察到显著的变化.
- 在实验室中,细菌HS修饰酶被证明可以减少SARS-CoV-2尖端蛋白结合和病毒感染.
- 工程Escherichia coli Nissle 1917有效地阻止了SARS-CoV-2的结合和人类细胞培养物的感染.
结论:
- 肠道开始性细菌具有修改HS的能力,从而影响肠道中的SARS-CoV-2易感性.
- 肠道粘膜的细菌理可能在调节病毒进入和感染方面发挥作用.
- 这些发现表明针对微生物相互作用的潜在治疗策略,用于控制病毒感染.
相关概念视频
Glycocalyx and its Functions
3.6K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
3.6K
Conjugated Proteins
18.2K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.2K
Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
GPCRs Regulate Adenylyl Cylase Activity
5.1K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.1K
Protein Glycosylation
6.7K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
6.7K
Yeast Signaling
14.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.3K

