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在酸盐饥饿和补充期间的裂变酵母代谢组动态

Ana M Sanchez^1,2, Aye K Kyaw³, Sara Nunes Violante³

¹Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.

|February 25, 2025

概括

裂变酵母中的酸盐饥饿会引发广泛的代谢转变,包括关键酸盐代谢物质的耗尽和营养物质利用的改变. 恢复发生在酸盐补充后,恢复细胞代谢和生长.

科学领域:

细胞代谢的细胞代谢.
营养物质的恒常性是营养的恒常性.
酵母生物学的酵母生物.

背景情况:

无机酸盐对细胞功能至关重要,影响许多代谢途径.
裂变酵母 (Schizosaccharomyces pombe) 作为研究酸盐平衡的模型.
以前的研究集中在酸盐饥饿期间的转录组和蛋白组变化.

研究的目的:

用代谢学来全面描述在裂变酵母中对酸盐饥饿的代谢反应.
为了研究在饥饿和恢复期间代谢物变化的时间动态.
促进对酸盐对细胞过程的影响的多学术理解.

主要方法:

在酸盐饥饿下对裂变酵母进行时间解析的代谢学概况.
在24小时的饥饿期内分析代谢物变化.
在补充后监测代谢恢复动力学.

主要成果:

酸盐饥饿导致NTPs,NDPs,dNTPs,共酶,糖解和酸途径中间体,核酸糖和脂前体的耗尽.
观察到早期克雷布斯循环中间体和贝他因的积累.
发生了新皮里米丁合成的阻断和S-adenosylmethionine的耗尽.
在酸盐补充后2-12小时内观察到代谢恢复和恢复生长.

结论:

裂变酵母在酸盐饥饿期间表现出广泛的代谢重编程,以保护酸盐.
代谢学提供了关于细胞适应营养压力的关键见解.
了解这些代谢变化对于理解细胞周期,基因表达和寿命调节至关重要.

关键词:

这种植物是Schizosaccharomyces pombe.酸盐的新陈代谢酸盐饥饿酸盐饥饿

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