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牛奶衍生的细胞外囊泡的糖光标记,用于研究它们的细胞吸收和细胞内命运

Xueqi Su1, Siqin Zhang1, Tianyu Zhang1

  • 1College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian, 361000, China.

Small methods
|February 25, 2025
PubMed
概括

一种新的糖甘定的光标签方法准确地跟踪牛奶衍生的细胞外囊泡 (mEVs). 这种技术克服了传统染料的局限性,使得对mEVs的吸收和药物输送进行精确的分析,用于治疗应用.

关键词:
药物输送是药物输送的过程.外基因组是外基因组的组成部分.细胞外囊泡中的细胞外囊泡.光标签上的光标签纳米流细胞计纳米流细胞计.

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...