在土耳其安卡拉的克拉里思素耐药的Helicobacter pylori:通过实时PCR检测在甲固定型氨酸嵌入的胃活检样本中
Betül Yüzügüldü1, Yusuf Üstün2, Sehbal Arslankoz3
1Ankara Etlik City Hospital, Department of Medical Microbiology, Ankara, Turkey.
The new microbiologica
|February 25, 2025
概括
在胃活检中发现了Helicobacter pylori (Hp) 的高克拉里素耐药率. 这表明标准的三重疗法不适合,需要区域性耐药性数据来进行有效的治疗计划.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 分子诊断学 分子诊断
背景情况:
- 杆菌 (Hp) 感染是胃炎和胃潰瘍的主要原因.
- 治疗HP的疗效在很大程度上取决于区域抗生素耐药性模式.
- 克拉里思素 (Cla) 是标准HP根除方案中的关键抗生素.
研究的目的:
- 在FFPE胃活检中确定Hp DNA和清红素耐药性 (ClaR) 的患病率.
- 评估HP感染患者的人口因素和ClaR之间的相关性.
- 根据观察到的抗性率,评估标准基于Cla的三重疗法的适用性.
主要方法:
- 从注入甲固定 (FFPE) 的胃活检中提取DNA.
- 聚合酶链反应 (PCR) 用于HPDNA检测和识别ClaR突变.
- 用更新的悉尼系统评估的胃活检样本用于组织病理学诊断.
主要成果:
- 在45.6% (68/149) 的胃活检样本中检测到hpDNA.
- 在42.6% (29/68) 的Hp阳性样本中发现了克拉里素耐药性 (ClaR).
- 与60岁以上的年龄组相比,在18-40岁的年龄组观察到更高的ClarR (OR5.5,p=0.03).
结论:
- 高分子确定的ClaR率 (42.6%) 表明标准三重疗法可能无效.
- 年龄似乎是影响克拉里胺耐药性的因素.
- 进一步的大规模区域研究对于指导HP治疗策略和抗生素管理至关重要.
更多相关视频
05:23Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
Published on: March 7, 2025
266
04:56Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis
Published on: May 16, 2025
98
相关概念视频
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
319
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
319
01:28Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
78
Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
78
02:57Real Time RT-PCR
56.7K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
56.7K