通过调节细胞外基质和自,CircTBCK可以预防骨关节炎
Human cell
|February 25, 2025
概括
这项研究将circTBCK确定为一种新型的循环RNA (circRNA),通过调节状细胞自,增殖和细胞外基质生产来保护骨关节炎 (OA). 上调circTBCK显示了对OA的治疗潜力.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 骨关节炎 (OA) 是一种常见的退行性关节疾病,没有有效的治疗方法.
- 循环RNAs (circRNAs) 越来越多地被认可为它们在OA病变发生中的作用.
- 在OA中circTBCK的特定功能仍然在很大程度上未被探索.
研究的目的:
- 研究新型circRNA,circTBCK在骨关节炎中的作用和机制.
- 确定circTBCK对状细胞活力,增殖,细胞外基质 (ECM) 生产和自的影响.
主要方法:
- 骨关节炎模型被建立在体内 (在小鼠中破坏介质月经手术的稳定性) 和体内 (对原发性肌肉细胞和ATDC5细胞的IL-1β治疗).
- 使用定量实时聚合酶连锁反应 (qRT-PCR) 和西式斑点测试来测量基因和蛋白质表达.
- 使用细胞计数套件-8 (CCK-8) 试验评估了慢性细胞活力,并通过免疫光 (IF) 评估了原II表达.
主要成果:
- 在OA模型中,circTBCK表达得到了改变.
- 在IL-1β治疗的冠状细胞中,circTBCK的过度表达增加了原II,SOX9,Ki-67,PCNA,LC3,BCl1和Atg5水平,同时降低了SQSTM1/P62.
- 破坏circTBCK加剧了IL-1β诱导的淋巴细胞退化.
结论:
- circTBCK通过调节状细胞自,增殖和ECM合成,在骨关节炎中起着保护作用.
- circTBCK代表了预防和治疗骨关节炎的潜在治疗标.
相关概念视频
The JAK-STAT Signaling Pathway
8.6K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.6K
Osteoclasts in Bone Remodeling
2.8K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
2.8K
Autophagy
4.1K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.1K
The Extracellular Matrix
8.8K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
8.8K
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K


