通过cGAS/STING/IRF3通路由SENP7诱导的微质质灭导致神经元灭
Lin Liu1, Fei Xiao1, Jinyue Yang1
1Department of Anesthesiology, Jiaxing Maternity and Child Health Care Hospital, Jiaxing Women and Children's Hospital, Wenzhou Medical University, Jiaxing 314050, Zhejiang, PR China.
Cytokine
|February 25, 2025
概括
通过增加SENP7,通过cGAS/STING/IRF3途径触发微质亡和神经元亡,孕产妇暴露于西沃弗兰会损害后代的认知能力.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 麻醉学 麻醉学
背景情况:
- 母亲接触麻醉剂可能会对发育中的胎儿带来神经认知风险.
- 连接母亲暴露于sevoflurane与后代认知缺陷的机制尚未完全理解.
研究的目的:
- 调查母体暴露于赛沃兰对胎儿大脑发育和后代认知功能的影响.
- 阐明底层的分子机制,重点关注SENP7和微质灭的作用.
主要方法:
- 怀孕的小鼠被暴露在sevoflurane;后代的认知功能被使用行为测试来评估.
- 在体外研究中评估了SENP7在微质亡和神经元亡中的作用.
- 分子测试 (西方斑,免疫沉) 分析了cGAS/STING/IRF3通路.
主要成果:
- 暴露于塞沃兰导致后代的认知障碍,蛋白质表达发生变化 (SENP7,Iba1,Caspase1,GSDMD-N, NeuN, TH).
- 在微质中,SENP7的敲击降低了热灭菌标记物和炎症性细胞因子 (IL-1β,IL-18).
- 对cGAS的SENP7脱SUMOylation激活了cGAS/STING/IRF3通路,诱导了微质质亡和神经元亡.
结论:
- 孕产妇暴露于塞沃兰会增加后代大脑中的SENP7,从而损害认知功能.
- 这种损伤是由SENP7调节的微质灭和随后通过cGAS/STING/IRF3通路的神经元灭介导的.
相关概念视频
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