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Updated: May 25, 2025

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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蛋白质工程用于空间时间解析的细胞监测

Guanwei Zhou1, Wenjing Wang1,2

  • 1Life Sciences Institute and Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan, USA; email: guzhou@umich.edu, wenjwang@umich.edu.

Annual review of analytical chemistry (Palo Alto, Calif.)
|February 25, 2025
PubMed
概括

蛋白质工程创造了基因编码的记者来跟踪活细胞中的细胞活动. 本综述涵盖了报告员的设计,评估以及生物监测中的蛋白质工程的未来机会.

关键词:
有基因编码的记者蛋白酶活动的蛋白酶活性.蛋白质工程工程 蛋白质工程蛋白质蛋白质相互作用信号分子的信号分子.时间空间分辨率

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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