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负压伤口疗法通过降低糖尿病足颗粒组织中的miR-155表达的调节来促进伤口愈合
Yixuan Huang1, Zhenyi Yu1, Murong Xu1
1Department of Endocrinology, the First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230032, Anhui, P.R. China.
Scientific reports
|February 25, 2025
概括
负压伤疗法 (NPWT) 在糖尿病足 (DFU) 颗粒组织中减少微RNA-155 (miR-155),促进愈合. 在DFU患者中,miR-155的减少与改善的伤口愈合率相关.
科学领域:
- 生物医学工程 生物医学工程
- 伤口治愈研究研究 伤口治愈研究
- 分子生物学分子生物学
背景情况:
- 糖尿病足 (DFU) 是一个重要的临床挑战,具有复杂的愈合过程.
- 微RNA-155 (miR-155) 已涉及与组织修复相关的各种细胞功能.
- 负压伤口治疗 (NPWT) 在调节DFU中miR-155等分子因子中的作用尚未完全理解.
研究的目的:
- 研究NPWT对DFU颗粒组织中miR-155表达的影响.
- 在DFU患者中探索miR-155水平变化与伤口愈合结果之间的相关性.
- 阐明负压在体外对miR-155表达和纤维细胞功能的影响.
主要方法:
- 一个随机对照试验,涉及60名DFU患者,将NPWT与例行服装进行比较.
- 定量实时PCR (qRT-PCR) 用于在治疗前一周和治疗后一周测量颗粒组织中的miR-155表达.
- 在体外研究中,使用正常的人体皮肤纤维细胞 (NHDFs) 暴露于负压 (-125 mmHg).
主要成果:
- 与基线相比,NPWT显著降低了DFU颗粒组织中的miR-155表达 (P <0.05).
- 在非NPWT组中没有观察到miR-155表达的显著变化 (P > 0.05).
- 在NPWT组中,miR-155表达的变化 (ΔmiR-155) 和伤口愈合率 (P = 0.028) 之间发现了正相关性.
- 在体外,负压降低了NHDFs中的miR-155表达,增强了增殖和迁移,同时降低了亡,部分是通过FGF7.7的上调调节.
结论:
- NPWT通过降低伤口颗粒组织中miR-155表达的调节,至少部分地促进DFU的愈合.
- 在DFU中NPWT的治疗疗效与miR-155水平的改变有关.
- 向miR-155调制是改善DFU愈合的潜在治疗策略.
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