碳流在经过工程改造的大肠杆菌中通过逆三碳酸循环途径在化学性质转化条件下重新连接
Jian-Hau Peng1,2,3,4, Shou-Chen Lo5, Yu-Ning Yu5,4
1Doctoral Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taichung City and Taipei City, 402 and 115, Taiwan.
Journal of biological engineering
|February 26, 2025
概括
工程化的大肠杆菌可以使用气固定二氧化碳 (CO2). 来自逆三碳酸循环的特定酶的表达使二氧化碳能够被纳入生物质,为可持续的碳捕获铺平了道路.
科学领域:
- 微生物学 微生物学
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 为直接将气态二氧化碳 (CO2) 纳入生物质而改造的大肠杆菌菌株.
- 利用气驱动的无氧呼吸和来自逆三碳酸 (rTCA) 循环的关键酶.
- 纳入的α-甲酸盐:来自Chlorobium tepidum的铁素氧化还原酶 (KOR) 和依赖ATP的酸酶 (ACL) 基因.
研究的目的:
- 研究KOR和ACL在工程E. coli中的二氧化碳同化中的作用.
- 为了确定在化学转化条件下的二氧化碳固定的最小酶要求.
- 分析代谢流量重定向及其对生物合成的影响.
主要方法:
- 大肠杆菌的基因工程以表达异构的rTCA循环酶 (KOR和ACL).
- 用作为电子捐赠体和二氧化碳作为碳源的化学石质培养.
- 同位素13CO2追踪用于追踪碳同化路径和代谢流量.
主要成果:
- 单独的KOR表达就支持在化学性质营养条件下的细胞维护.
- ACL与KOR的同时表达显著增强了二氧化碳同化和生物质生产.
- 同位素标记证明了KOR在二氧化碳固定到TCA代谢物中的作用,而ACL则将流量重定向到氨基酸和核酸生物合成.
结论:
- 通过使用最小的异质酶来设计大肠杆菌的二氧化碳固定的新策略.
- 揭示了对二氧化碳吸收的遗传和营养要求的见解.
- 突出了可持续碳固定和生物技术应用的潜力.
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