猪流行性腹病毒中的尖端基因变异性作为毒性决定因素
Wentao Li1, Basav N Hangalapura2, Paul van den Elzen2
1Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
Journal of virology
|February 26, 2025
概括
猪流行性腹病毒 (PEDV) 尖峰基因的遗传变异显著影响病原性. 高毒性尖基因导致小猪的严重疾病和死亡,突出突出了尖蛋白在PEDV毒性中的作用.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子病原体的产生.
- 猪的传染病 猪的传染病
背景情况:
- 猪流行性腹病毒 (PEDV) 导致严重的肠道疾病和猪仔的高死亡率.
- PEDV菌株表现出基因型变异,特别是在 (S) 基因中,这会影响病毒性.
- S蛋白对于病毒的进入至关重要,也是免疫系统的关键目标.
研究的目的:
- 调查尖峰 (S) 基因变异对猪流行性腹病毒 (PEDV) 病原性的直接影响.
- 在猪模型中阐明不同S基因变异在疾病严重性和死亡率中的作用.
主要方法:
- 使用向RNA重组生成复合PEDV (rPEDV) 变种的生成.
- 从当代毒性菌株 (UU和GDU) 的尖基因纳入毒性DR13疫苗菌株背景.
- 在用工程rPEDV菌株接种的3天大的猪中对病原性和病毒分泌的评估.
主要成果:
- 带有DR13尖峰基因的RPEDV是非致病的.
- 带有UU尖峰基因的RPEDV诱导了轻度至重度的腹,有中度的病毒泄露,但没有死亡率.
- 具有GDU尖峰基因的RPEDV导致严重腹,高病毒标位和高死亡率.
结论:
- 尖端蛋白质是PEDV毒性的关键决定因素.
- 在PEDV尖峰基因的变异直接与疾病的严重程度和死亡率相关.
- 了解S基因变异性对于开发有效的PEDV控制策略和疫苗至关重要.
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