阿格拉稀释与汁稀释可比,用于在Streptococcus agalactiae中确定MIC
Edward A R Portal1,2,3, Caitlin Farley1, Teresa Iannetelli1,2
1Division of Infection and Immunity, Department of Medical Microbiology, University Hospital of Wales, Cardiff University, Cardiff CF14 4XN, UK.
Antibiotics (Basel, Switzerland)
|February 26, 2025
概括
阿格拉稀释测试提供了一种高通量方法,用于监测B组链球菌 (GBS) 的抗菌素耐药性. 这种方法对于追踪新出现的耐药性趋势和指导治疗至关重要,尽管不是用于例行诊断.
科学领域:
- 临床微生物学 临床微生物学
- 传染性疾病 传染性疾病
- 抗微生物药物耐药性的监测和监测
背景情况:
- B组链球菌 (GBS) 导致新生儿败血症,随着宏化物/林科萨米德耐药性增加,限制了对青素过敏个体的选择.
- 在GBS中增加抗菌素耐药性 (AMR) 需要强有力的监测方法.
- 目前的微稀释 (BMD) 方法不适合进行大规模的抗菌素敏感性测试 (AST).
研究的目的:
- 为了验证阿加稀释AST作为GBS的BMD的替代品.
- 评估阿加稀释剂对GBS抗菌敏感性的高通量查的能力.
主要方法:
- 对24种GBS菌株的阿加稀释和微稀释AST与8种抗生素进行比较.
- 评估的最小抑制度 (MIC) 协议和耐药性概况的相关性使用科恩的kappa.
- 评估了阿加稀释剂在检测尾随生长和污染物的性能.
主要成果:
- 对大多数测试的抗微生物药物来说,阿格拉稀释显示>90%的MIC与BMD一致.
- 观察到强烈的一致性 (kappa 0.88-1.00) 对抗性的确定.
- 与BMD相比,agar稀释减轻了尾行生长问题,并改善了污染物的检测.
结论:
- 阿加稀释是一种有效的,高通量方法,用于GBS中的AMR监测,支持趋势识别和指导方针开发.
- 该方法适用于分析大型回顾性队列 (96个隔离物/盘).
- 由于每种抗微生物药物所需的板数,阿格拉稀释对于常规的临床诊断是不切实际的.
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