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High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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用UHPLC-HRMS/MS进行中性大麻素量化的优化提取方法.

João Victor Meirelles1,2, Débora Cristina Diniz Estevam2, Vanessa Farelo Dos Santos2

  • 1Departamento de Química, Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro 22451-045, RJ, Brazil.

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概括
此摘要是机器生成的。

对大麻草本提取物 (CHE) 的优化提取方法改善了对CBD,THC和CBN等关键大麻素的分析. 这种更快,更便宜,更可靠的方法确保了对人用和兽药药品质的准确药品质量控制.

关键词:
盒子Behnken设计设计大麻草本提取物 大麻草本提取物完全的工厂设计设计.在UHPLC-HRMS/MS中使用.大麻素是一种大麻素.

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科学领域:

  • 分析化学 分析化学
  • 药理学是指药理学,即药理学是指药理学.
  • 法医科学 法医科学 法医科学

背景情况:

  • 全球大麻市场正在增长,在临床应用中对大麻草本提取物 (CHE) 的需求正在增加.
  • 像大麻 (CBD),四大麻 (THC) 和大麻 (CBN) 这样的中性大麻素是CHE的关键活性化合物.
  • 在CHE中非标准化的大麻素含量给药品质量控制和治疗疗效带来了挑战.

研究的目的:

  • 开发和优化一种用于从商业CHE中提取CBD,THC和CBN的多变量方法.
  • 通过响应表面方法验证预测模型,以提取效率.
  • 建立一个快速,经济有效和可靠的分析方法,用于CHE中大麻素量化.

主要方法:

  • 使用全因数和盒子-Behnken设计来优化提取参数.
  • 采用甲醇/ (9:1 v/v) 溶剂系统,体积最小,提取时间短.
  • 使用超高性能液体色谱学与高分辨率联质谱学 (UHPLC-HRMS/MS) 结合使用量化大麻素.

主要成果:

  • 激发和超声波的时间被确定为关键的提取参数,其次是溶剂体积.
  • 优化的方法显示了高精度 (<15%),准确性 (69-98%),灵敏度 (23-39 ng kg-1),和线性.
  • 在3小时的时间内成功处理了16个商业CHE样本,证明了实用性和可重复性.

结论:

  • 与现有的文献方法相比,开发了一种更快,更便宜,更有效的加拿大麻素提取协议.
  • 使用UHPLC-HRMS/MS实现了最先进的分析灵敏度和特异性,用于可靠的常规分析.
  • 经过验证的方法适用于商业CHE在人类和兽医药物应用中的质量控制.