相关实验视频

Updated: May 5, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

TRPC6-介导的Zn2+输入负面调节血管光滑肌细胞的收缩分化

Chenlin Su¹, Xinya Mi¹, Tomoya Ito¹

¹Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.

|February 26, 2025

概括

通过TRPC6通道的流入调节了血管光滑肌细胞 (VSMC) 现型切换. 这一发现表明TRPC6介导的进入是血管重塑疾病的潜在治疗标.

科学领域:

心血管生物学心血管生物学
细胞生理学细胞生理学
分子医学是分子医学.

背景情况:

血管光滑肌细胞 (VSMC) 表现出表型可塑性,在收缩和合成状态之间切换,这对血管健康和疾病至关重要.
规范性短暂受体潜力6 (TRPC6) 通道和细胞内 (Zn2+) 已与VSMC功能和心血管影响有关.

研究的目的:

研究TRPC6介导的Zn2+流入在VSMC表型切换和血管改造中的作用.
为了确定TRPC6依赖的Zn2+流入是否影响转化生长因子β (TGFβ) 诱导的VSMC变化.

主要方法:

已建立的老鼠大动脉光滑肌细胞 (RAoSMCs),表达野生类型 (WT) 或一种Zn2+无透的TRPC6突变 (KYD).
利用TRPC6的药理激活与PPZ2和TGFβ刺激来评估细胞内Zn2+水平和VSMC分化.
检查了TRPC6依赖的阴离体电流,并评估了PPZ2对小鼠血管新生素II诱导的血管改造的影响.

主要成果:

通过PPZ2激活TRPC6,阻止了TGFβ诱导的细胞内Zn2+的减少和WT RAoSMCs的收缩分化的丧失,但不是KYD RAoSMCs.
不管VSMC表型如何,TRPC6通道都在功能上表达,因为离子电流保持一致.
在接受慢性血管激素II输液的小鼠中,PPZ2治疗减弱了血管改造.

结论:

通过TRPC6通道的Zn2+流入会负面调节TGFβ诱导的VSMC的收缩分化.
通过TRPC6介导的Zn2+流入在缓解血管改造过程中起着重要作用.
针对TRPC6依赖的Zn2+通路可能为血管疾病提供一种新的治疗策略.

关键词:

在 TRPC6 频道中.Zn2+ 涌入的流量现型切换的切换方式血管光滑肌肉细胞的细胞.

更多相关视频

Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

Published on: March 4, 2020

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

Published on: June 2, 2023

相关概念视频

Cell Polarization by Rho Proteins

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...

Smooth Muscle Contraction

Smooth Muscle Contraction

Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...

Antihypertensive Drugs: Action of Calcium Channel Blockers

Antihypertensive Drugs: Action of Calcium Channel Blockers

Calcium ions are essential to contract smooth muscle cells in blood vessels. They enter these cells through voltage-dependent calcium channels, specifically L-type calcium channels in the cell membrane. These L-type calcium channels are integral to the excitation-contraction coupling process in smooth muscle. When a stimulus is received by smooth muscle cells, their membrane depolarizes. This alteration in membrane potential instigates the opening of L-type calcium channels. As a result,...