相关实验视频

Updated: May 25, 2025

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

Published on: August 14, 2015

诱导性牙细菌的分泌体表现出牙发育所需的信号

Anahid A Birjandi¹, Paul Sharpe¹

¹Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, King's College London, London SE1 9RT, UK.

Bioengineering (Basel, Switzerland)

|February 26, 2025

概括

研究人员探索了牙生殖细胞,特别是小细胞外囊泡 (sEVs) 的信号是否可以帮助牙发育. 他们发现,介质细胞中的SEVs会影响表皮细胞中的基因表达,这表明它在牙形成中的作用.

科学领域:

发展生物学发展生物学
细胞生物学细胞生物学
再生医学是一种再生医学.

背景情况:

牙的发育依赖于上皮细胞和介质细胞之间的复杂信号传递.
以前的生物工程尝试受到体外细胞扩张问题的限制.
牙发育所需的关键诱导信号在很大程度上仍未被确定.

研究的目的:

为了研究牙发育中的诱导性牙胚芽介质中的分泌体和小细胞外囊 (sEVs) 的作用.
为了确定sEV是否可以提供牙形成所需的感应信号.
分析sEVs对牙生殖细胞的蛋白质学和转录学影响.

主要方法:

净化和特征小细胞外囊泡 (sEVs) 和从诱导性牙胚芽介质体的分泌体.
在不同培养条件下对sEVs和介质细胞进行蛋白质组分析 (2D与3D,持续时间).
RNA测序 (RNASeq) 用于评估用sEVs治疗的牙胚芽上皮细胞的转录组变化.

主要成果:

培养时间和环境 (2D/3D) 显著影响净化SEVs的蛋白质组.
对于sEVs独特的蛋白质与关键牙发育信号通路有关.
用sEVs治疗增加了在上皮细胞中已知的odontogenic基因的表达.

结论:

来自牙胚芽介质的小细胞外囊 (sEVs) 有助于牙发育所需的诱导信号.
sEVs可以通过调节细胞代谢和细胞外矩阵组织来影响牙的形成.
对sEV的进一步研究有可能为再生牙科和生物工程新牙提供潜力.

关键词:

生物牙生物牙是一种生物牙.无细胞组织再生组织再生.一些秘密一些秘密.小小的细胞外囊泡.牙再生牙再生

更多相关视频

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

Published on: November 13, 2013

A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals

A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals

Published on: February 14, 2020

相关概念视频

Teeth

Teeth

The formation of teeth, also known as odontogenesis, is a complex process that begins in utero, around the sixth week of embryonic development. There are three stages to this process: the bud stage, the cap stage, and the bell stage.
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...

Tooth Anatomy

Tooth Anatomy

The human tooth enables us to eat a variety of foods, speak clearly, and even aid in shaping our faces. Teeth are composed of various elements that work together. Here's a detailed look at the anatomy of a human tooth.
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or...

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...