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Updated: May 25, 2025

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

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在3D打印模具启用的水凝中开放微流体细胞培养.

Madison O'Brien^1,2, Ashley N Spirrison¹, Melati S Abdul Halim³

¹Jim and Joan Bock Department of Biomedical Engineering, Trine University, Angola, IN 46703, USA.

Bioengineering (Basel, Switzerland)

|February 26, 2025

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研究人员开发了用于开放微流体细胞培养的3D打印模具,创造了类似血管的组织模拟器. 这种简单的方法提高了体外研究和疾病机制研究的可访问性.

科学领域:

生物材料工程生物材料工程
组织工程是组织工程.
微流体学微流体学

背景情况:

试管细胞培养模型对于研究细胞行为和疾病机制至关重要.
现有的微流体系统可能很复杂,需要专门的设备.
需要可访问,多功能细胞培养平台,模仿生理结构.

研究的目的:

开发一种使用3D打印模具创建开放微流体细胞培养的简化方法.
在不同的水凝中展示各种模仿组织结构的制造.
验证这些结构用于体外生物研究的使用,包括模拟血管.

主要方法:

使用3D打印模具,在水凝中创建开放的微流体通道和井 (糖,凝, I型原).
制造的开放道,直径不同 (400微米至4毫米) 和原密度不同 (2-4毫克/毫升).
在开放道中使用人类静脉内皮细胞 (HUVEC) 模仿生成的血管并将其置于缺氧状态.

主要成果:

在多个水凝中成功制造了多种几何形状,包括开放道和各种井形状.
在直径为800微米和2毫米的血管模拟器中实现了高细胞活力 (>89%).
与normoxia相比,在低氧条件下观察到细胞活力 (8.3%) 和CD31表达 (7.4%) 的显著降低.

关键词:

3D打印的模具可以使用3D打印.血液血管模仿的模仿原蛋白是一种原蛋白.这是一种水凝.开放式的微流体学

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结论:

3D打印模具方法提供了一种更简单,更容易获得的方法来创建多功能 in vitro 组织模拟器.
这种技术可以生成生理学上相关的结构,用于研究细胞对各种条件的反应,例如缺氧.
开发的平台有望推动细胞生物学,疾病建模和药物发现的体外研究.