在预发酵过程中生物氨基的发酵定律通过相关性分析揭示出来
Lijing Liu1,2, Jinyu Zhao1, Dapeng Lu1
1College of Food Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Kunming 650201, China.
Foods (Basel, Switzerland)
|February 26, 2025
概括
发酵豆 (sufu) 中的生物氨基酸 (BA) 在发酵过程中减少,受水含量,蛋白质含量,pH值和质感的影响. 这项研究澄清了苏夫安全的BA形成机制.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 生物化学 生化学
背景情况:
- 苏福是一种传统的中国发酵豆,由于蛋白质水解产生氨基酸和生物氨基酸 (BA),它具有独特的乌玛米味道.
- 食物中BA含量升高可能会导致不良健康影响,包括头痛和消化问题,需要了解它们在发酵产品中的形成.
- 在苏夫发酵过程中产生BA的精确机制尚未完全阐明.
研究的目的:
- 为了研究在苏夫的发酵过程中生物氨基 (BA) 含量的动态变化.
- 探索物理化学特性 (质地,水含量,可溶蛋白,pH) 和BA形成之间的关系.
- 为了阐明 BA 生产的基础机制,在用 *Mucor racemosus* 发酵的苏夫中进行 BA 生产.
主要方法:
- 在不同时间间隔分析了用*Mucor racemosus*发酵的苏福样本.
- 使用高性能液态染色体质谱法 (HPLC-MS) 来量化生物性氨基酸.
- 在整个发酵过程中测量了质地,物理化学性质和BA含量.
主要成果:
- 在发酵过程中,生物氨基的总含量从60.66降至38.19毫克/公斤.
- 精子胺,精子胺和尸体胺是被确定的主要BA.
- BA水平与水含量呈正相关,与可溶蛋白呈负相关;低pH抑制了BA的形成,而柔软的质地则促进了 BA的形成.
结论:
- 该研究成功地描述了在苏夫发酵过程中生物氨基胺的动态变化和形成机制.
- 研究结果表明,含水量,可溶性蛋白质,pH值和质地显著影响水中BA水平.
- 这项研究通过了解BA动态,为确保Moudingsufu的安全和质量控制提供了关键的见解.
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