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关于识别侵袭性Bactericera cockerelli (Hemiptera: Triozidae) 捕食者群体的初步研究和开发用于测试野外捕食者的分子识别工具的初步研究

Shovon Chandra Sarkar¹, Stephen Paul Milroy¹, Wei Xu¹

¹Food Futures Institute, Murdoch University, Murdoch, WA 6150, Australia.

|February 26, 2025

概括

西澳大利亚的掠食性昆虫捕食入侵的番茄和马psyllid,Bactericera cockerelli. DNA分析证实,45%的捕食者,特别是甲虫,吃过这种害虫.

科学领域:

昆虫学昆虫学是一门学科.
虫害管理虫害管理虫害管理
分子生态学分子生态学

背景情况:

番茄土豆 (Bactericera cockerelli) 是影响Solanaceae作物的主要害虫.
B. cockerelli于2017年首次在西澳大利亚被发现,对农业构成新的威胁.
了解自然捕食者复合体对于管理入侵物种至关重要.

研究的目的:

为了识别西澳大利亚Solanaceae领域的入侵性番茄土豆虫,Bacteriicera cockerelli的捕食者.
评估本地掠食者社区在控制B. cockerelli种群中的作用.
开发和应用分子工具来识别捕食者与猎物的相互作用.

主要方法:

在关键农业地区使用扫网对掠食性昆虫和蜘蛛进行实地采样.
实验室养试验以优化聚合酶链反应 (PCR) 起始剂用于检测B. cockerelli DNA.
使用PCR对现场采集的捕食者进行查,以确定那些消耗了B. cockerelli.

主要成果:

收集了各种各样的掠食性类型,其中绿色蕾丝翅和蝶是最丰富的.
通过PCR分析证实了B. cockerelliDNA在7种已识别的种群中的45%采样的掠食性昆虫中.
科乐类 (甲虫) 捕食者表现出B. cockerelli DNA的阳性结果的频率最高.

结论:

这项研究提供了第一个基于现场的证据,证明澳大利亚本土的捕食者食用了侵入性的B. cockerelli.
居民昆虫捕食者,特别是甲虫,在自然控制B. cockerelli.中发挥着重要作用.
在对侵袭性害虫的生态研究中,分子方法有效地识别了捕食者-猎物关系.

关键词:

一般主义的捕食者捕食者侵袭性害虫侵袭性害虫是一种入侵性的害虫.分子检测分子检测检测捕食掠食掠食掠食捕食者猎物的关系关系

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