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对低温应激反应中Juglans regia的转录组和微RNA分析

Haochang Zhao^1,2, Xia Luo^1,2, Caihua Guo^1,2

¹College of Agriculture, Shihezi University, Shihezi 832003, China.

International journal of molecular sciences

|February 26, 2025

概括

核桃苗在低温压力下表现出生理变化,发现了显著的基因表达和微RNA变化. 这项研究提供了对面临晚损害的核桃寒冷耐受机制的见解.

科学领域:

植物生理学植物生理学
分子生物学分子生物学
农业科学农业科学

背景情况:

核桃是全球重要的粮食和油作物.
在高度地区的晚损害会导致核桃苗的显著低温压力.
了解耐寒性分子机制对于核桃种植至关重要.

研究的目的:

在低温 (LT) 压力下调查核桃苗的生理和生化变化.
阐明潜在的分子机制,包括基因和microRNA表达,以应对LT压力.

主要方法:

核桃幼苗在48小时内接受了LT压力 (4°C).
测量了生理指数 (电导率,甲,Fv/Fm).
转录组和microRNA测序在不同的时间点在叶片样本上进行.
进行了生物信息分析,包括基因本体学 (GO) 和基因和基因组 (KEGG) 丰富的京都百科全书.
实时光定量PCR (qRT-PCR) 用于验证.

主要成果:

LT应力增加了电导率和含量,同时降低了Fv/Fm.
鉴定了12566个差异表达基因 (DEG),这些基因在多糖代谢和烯胺生物合成中得到丰富.
确定了1052个微RNA (miRNA),目标是7043个基因.
转录组和miRNA数据的整合揭示了244种不同的DEGs参与了像粉和糖代谢这样的代谢途径.
用qRT-PCR验证了基因表达模式.

结论:

低温压力显著影响了核桃苗的生理和分子通路.
不同基因和miRNA表达在核桃对寒冷压力的反应中起着关键作用.
这项研究提供了宝贵的遗传资源,用于增强核桃对晚损害的耐寒性.

关键词:

朱格兰斯·雷吉亚 (Juglans regia) 是一个皇家法官.在RNA-seqqq.低温应力压力低温应力压力这是一个微型RNA.

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