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应用mRNA-Seq和元基因组测序来研究Salmonella pullorum在的感染

Xiaohuan Chao¹, Zhexia Fan^1,2, Jiongwen Wu^1,2

¹State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou 510642, China.

International journal of molecular sciences

|February 26, 2025

概括

沙门氏菌 (Salmonella pullorum) 感染显著改变的基因表达和肠道微生物群. 这项研究探讨了基因和微生物层面的这些变化,以了解疾病.

科学领域:

禽类的健康和疾病
基因组学和转录基因组学
微生物组研究微生物组研究

背景情况:

沙门氏菌 (Salmonella pullorum) 感染导致家禽大量死亡和经济损失.
关于沙门氏菌 (Salmonella pullorum) 对骨基因表达和肠道微生物群的影响的研究有限.
了解这些相互作用对于减轻家禽疾病影响至关重要.

研究的目的:

为了研究沙门氏杆菌 (Salmonella pullorum) 感染对的基因表达的影响.
分析在感染沙门氏菌 (Salmonella pullorum) 后肠道微生物群组成的变化.
探索在Salmonella pullorum感染期间基因转录和微生物变化之间的关系.

主要方法:

mRNA测序 (mRNA-Seq) 在受感染和对照的头组织上进行.
使用元基因组测序来分析新鲜便中的微生物组.
进行了差异基因表达 (DEG) 和替代拼接 (DEAS) 分析.

主要成果:

鉴定了1560个差异表达基因 (DEGs),其中380个是上调调的,1180个是下调调的.
70个基因显示出差异性表达和替代拼接,包括TPM1和SPPL2A.
沙门氏菌 (Salmonella pullorum) 感染显著改变了主要细菌族群的丰富性:细菌群 (Bacteroidetes),细菌群 (Firmicutes) 和细菌群 (Verrucomicrobia).

结论:

这项研究提供了在中Salmonella pullorum感染的遗传和微生物基础的初步探索.
像TPM1和SPPL2A这样的基因与宿主对感染的反应有关.
在Salmonella pullorum感染期间,肠道微生物组合发生了显著的变化.

关键词:

这就是DeASs.这些DEGs是DEGs.沙门氏菌 (Salmonella pullorum) 是一种有毒的植物.这些都是肉肉.微生物社区的微生物社区.

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