无细胞核酸用于急性缺血性中风的早期诊断:系统性审查和元分析
Xiaodan Zhang1, Yuee Cai1, Brian Hon Man Sit1
1Department of Emergency Medicine, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
International journal of molecular sciences
|February 26, 2025
概括
识别急性缺血性中风 (AIS) 的早期诊断生物标志物至关重要. 无细胞DNA (cfDNA) 和特定的微RNA显示出在72小时内快速诊断AIS的希望.
科学领域:
- 生物化学 生物化学
- 遗传学 遗传学 是一个
- 神经学 神经学
背景情况:
- 快速识别急性缺血性中风 (AIS) 对于有效治疗至关重要.
- 现有的诊断方法可能耗时,延迟关键干预.
- 在无细胞核酸 (cfNAs) 中的生物标志物为更快的诊断提供了潜力.
研究的目的:
- 确定最有前途的无细胞核酸 (cfNA) 作为AIS的诊断生物标志物.
- 在症状发作后72小时内评估cfNAs以诊断AIS.
- 综合关于cfNAs在早期AIS检测中的诊断性能的证据.
主要方法:
- 在PubMed,科学网,EMBASE和科克兰图书馆的系统文献搜索,截至2023年5月10日.
- 包括评估血液cfNAs用于早期AIS诊断的研究.
- 随机效应的元分析,以基于AIS和非AIS患者之间的折叠变化汇集诊断性能.
主要成果:
- 66篇文章报告了143个不同的cfNA,从2955个记录中满足了包含标准.
- 高质量的研究确定了cfDNA,miR-106b-5p,miR-124,miR-155和lncRNA H19作为有前途的生物标志物.
- 从四项研究中对cfDNA的元分析 (355名AIS患者,97名对照) 显示了显著的折叠变化 (汇总比率1.48,p < 0.001).
结论:
- cfDNA,miR-106b-5p,miR-124,miR-155和lncRNA H19是早期AIS诊断中最有前途的生物标志物.
- 这些cfNA显示了急性缺血性中风的快速识别的潜力.
- 需要进一步的研究来验证这些生物标志物在AIS诊断中的临床应用.
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