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实验室冷软X射线显微镜的样品制备协议用于研究细胞纳米粒子吸收

Komang G Y Arsana1, Martin Svenda1, Hans M Hertz1

  • 1Biomedical and X-Ray Physics, Department of Applied Physics, KTH Royal Institute of Technology, 10691 Stockholm, Sweden.

International journal of molecular sciences
|February 26, 2025
PubMed
概括

本研究介绍了用于实验室软X射线显微镜 (LSXM) 的样本制备协议,以可视化细胞纳米粒子吸收. 优化的玻璃化确保了薄,高对比度的样本,以改善生物结构的成像.

关键词:
在X射线显微镜中使用X射线显微镜.细胞成像细胞成像结冷固化 结冷固化纳米颗粒是一种纳米粒子.样品的准备 样品的准备窗户的水,窗户的水

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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