miR-212-5p通过向LAMC2和LAMA3来调节PM2.5诱导的亡
Yunna Jia1, Xiqing Zhang1, Cuizhu Zhao1
1Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
International journal of molecular sciences
|February 26, 2025
概括
细颗粒物 (PM2.5) 暴露会诱导肺细胞亡. 微RNA-212-5p针对LAMC2和LAMA3,调节细胞死亡途径,并提供有关PM2.5肺损伤的新见解.
科学领域:
- 环境健康 环境健康
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 细颗粒物 (PM2.5) 暴露与呼吸系统疾病和细胞损伤有关.
- 在PM2.5引起的肺损伤中观察到微RNA (miRNA) 表达变化,但它们的功能尚不清楚.
研究的目的:
- 研究微RNA-212-5p (miR-212-5p) 在PM2.5诱导的亡中的作用.
- 为了确定miR-212-5p在应对PM2.5暴露时调节的分子标和途径.
主要方法:
- 双露西法酶记者测定证实miR-212-5p.p.直接准LAMC2和LAMA3.
- 分析了miR-212-5p,LAMC2,LAMA3和亡之间的调节关系.
- 研究PI3K-AKT和NF-κB信号通路的参与.
主要成果:
- miR-212-5p直接准了拉米因子单元LAMC2和LAMA3.
- LAMC2和LAMA3正调节PI3K-AKT通路,负调节NF-κB通路. LAMC2和LAMA3正调节PI3K-AKT通路,负调节NF-κB通路.
- miR-212-5p通过PI3K/AKT/NF-κB通路准LAMC2和LAMA3来调节亡速度.
结论:
- 揭示了一个新的分子机制,其中miR-212-5p针对LAMC2和LAMA3,影响PM2.5-诱导的亡.
- 这项研究重新定义了miR-212-5p在亡中的作用及其与环境污染物的联系.
- 这些发现为了解和潜在地治疗PM2.5相关的肺病理提供了新的途径.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Master Transcription Regulators
6.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K
Regulation of Expression at Multiple Steps
864
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
864
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Regulation of Expression Occurs at Multiple Steps
22.4K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.4K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K


