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在Apis cerana幼虫中的miRNAs的转录基因表征 响应Ascosphaera apis感染
Yuxuan Song1, Jianfeng Qiu1,2,3, Jing Kang1
1College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Genes
|February 26, 2025
概括
这项研究揭示了microRNAs (miRNAs) 如何调节亚洲蜜蜂 (Apis cerana cerana) 针对由Ascosphaera apis.引起的粉笔虫病的免疫反应. 像ace-miR-539-y和ace-miR-1277-x这样的关键miRNA被确定为早期和晚期免疫防御的关键.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 昆虫学 昆虫学是一门学科.
- 菌类学 菌类学是指菌类学.
背景情况:
- Ascosphaera apis的感染会导致粉笔病,导致蜜蜂群落下降.
- 微RNA (miRNA) 调节在蜜蜂对A. apis免疫力的特定作用仍然不太清楚.
研究的目的:
- 在Apis cerana幼虫中识别分化表达的miRNAs (DEmiRNAs),这些幼虫对A. apis感染有反应.
- 分析这些DEmiRNAs的调节网络和功能.
- 阐明miRNA介导的免疫反应机制,以对抗A. apis.
主要方法:
- 小RNA测序 (sRNA-seq) 用于在不同时间点 (AcT1,AcT2,AcT3) 在受感染的蜜蜂肠道中描述miRNA表达.
- 茎环RT-PCR被用于验证选定的DEmiRNAs的表达.
- 对目标mRNA进行了生物信息分析,包括基因本体学 (GO) 和KEGG通路丰富.
- 建立了miRNA-mRNA调节网络.
主要成果:
- 总共有537个miRNA被确定,分别在AcT1,AcT2和AcT3检测到10,27个和54个DEmiRNA,随着时间的推移,这些数字增加了.
- 经验证的DEmiRNA显示出与sRNA-seq数据一致的表达趋势.
- 丰富的GO术语包括结合和细胞过程;KEGG途径涉及内细胞酶,蛋白质分解,体和JAK-STAT信号.
- ace-miR-539-y和ace-miR-1277-x分别在早期和晚期免疫反应中被确定为核心miRNA,分别在JAK-STAT和细胞体通路内.
结论:
- 微RNA在蜜蜂对A. apis感染的免疫反应中发挥着重要作用.
- miRNA调节影响宿主能量代谢,细胞免疫和幽默免疫.
- 这项研究为Apis cerana cerana免疫和宿主-病原体相互作用中的miRNA调节提供了基础的见解.
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