在酵母中响应乙醇压力的长非编码RNA似乎与蛋白质合成和膜完整性有关
Amanda Piveta Schnepper1, Agatha M S Kubo2, Camila Moreira Pinto1
1Department of Bioprocess and Biotechnology, School of Agriculture, Sao Paulo State University (UNESP), Botucatu 18610-034, SP, Brazil.
Genes
|February 26, 2025
概括
酵母中的乙醇应激反应涉及长非编码RNA (lncRNAs),影响蛋白质合成和有机体组装. 这些应激反应的lncRNAs,transcr_9136和transcr_10027,为酵母耐受性和工业应用提供了洞察力.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 生物化学 生物化学
背景情况:
- 细胞应激监测涉及翻译和无膜有机体的形成.
- 响应乙醇压力的长非编码RNA (lncRNAs),特别是transcr_9136和transcr_10027,与酵母乙醇耐受性有关.
- 调查这些lncRNAs,乙醇压力,蛋白质生物发生和有机细胞组装之间的联系至关重要.
研究的目的:
- 确定是否对乙醇反应的lncRNAs (transcr_9136和transcr_10027) 与乙醇应激下蛋白质生物发生和无膜有机体组合有关.
- 阐明SEY6210菌株中的transcr_9136和BY4742菌株中的transcr_10027在乙醇应激反应方面的特定作用.
- 探索这些应激反应性 lncRNAs 在乙醇生产等过程中的潜在工业应用.
主要方法:
- 在乙醇压力下的野生类型和淘汰酵母菌株中对lncRNAs和相关基因 (ILT1,RRP1,27S,25S,TIR3,FAA3) 的基因表达分析 (qPCR).
- 对关键翻译因子 (DCP1a,PABP,eIF4E) 的蛋白质水平评估 (西式涂抹).
- 生物信息学分析预测lncRNA调节相互作用 (transcr_9136-RRP1,transcr_10027-Tel1p),细胞死亡率评估 (流细胞计) 和总蛋白产量评估.
主要成果:
- 在SEY6210中的transcr_9136控制RRP1和27SrRNA的表达,减少一般翻译,并影响细胞膜完整性.
- 在 BY4742 中的 transcr_10027 抑制了处理体的形成,并增加了一般的转化水平.
- 这两种lncRNA都在蛋白质合成和细胞对乙醇压力的反应中发挥着不同的调节作用.
结论:
- 这项研究提供了第一个证据,表明lncRNAs在压力期间影响酵母蛋白合成和无膜有机细胞组装.
- 已经确定了酵母中应激反应性lncRNAs的新机制.
- 这些发现表明lncRNAs在优化基于酵母的发酵过程中的潜在工业应用,例如增强乙醇生产.
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