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Updated: May 25, 2025

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菌体甲基酶中的一个主动定位插入元件含有隐藏的HNH内核酶

Danielle Arsenault¹, Sophia P Gosselin¹, Johann Peter Gogarten^1,2

¹Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06268-3125, USA.

|February 26, 2025

概括

一个新的菌体插入元件,ShiLan域,被确定为一个外的histidine-asparagine-histidine (HNH) 内核酶,而不是一个intin或intron. 这一发现扩大了对细胞菌体中移动遗传元素和重叠阅读框架的理解.

科学领域:

遗传学是一个遗传学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

在菌体DNA甲基酶基因中发现的ShiLan域最初被认为是因进化模式而形成的整体或内基.
然而,它缺乏自我拼接域,也没有产生与宿主基因相对应的内部停止编码子.
这表明它可能是一个不寻常的intin或非intronic元素.

研究的目的:

为了确定ShiLan域插入的真实性质.
调查其潜在的功能和进化意义.
探索其他生物体中类似元素的流行和翻译机制.

主要方法:

在ShiLan插入序列中对交替开放的读取进行计算分析.
在菌体和 prokaryotic 基因组中进行序列相似性搜索.
与宿主甲基酶基因相框插入的结构预测.

主要成果:

该ShiLan域被确定为一个框架外的histidine-asparagine-histidine (HNH) 内核酶,由GTG编码器启动并由TAA编码器终止.
没有证据支持其被归类为已知的内因或内因.
预计插入将形成循环结构,可能避免干扰DNA甲基酶活性.
在其他菌体和 prokaryotes 中发现了同源的外框架内核酶元素.

结论:

希兰域代表了一种新型的移动遗传元素.
这些发现突显了菌体中重叠的开放阅读框架的流行和多样化的安排.
这扩大了对非正统的遗传元素及其对归属的翻译策略的理解.

关键词:

它们的作用是actinobacteriophage.结核酶 (endonuclease) 的作用是什么在家里找回来.自私的遗传元素自私的遗传元素

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