从Panax notoginseng叶子中分离,净化,结构特征和多糖的体外低血糖活性
Xueling Zhang1, Chongying Shi1, Zilin Wang1
1College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Molecules (Basel, Switzerland)
|February 26, 2025
概括
这项研究优化了Panax notoginseng叶多糖 (PNLP) 的提取,并发现纯化的分量PNLP-3有效抑制消化酶并改善葡萄糖代谢. 通过减少氧化应激和增强葡萄糖吸收,PNLP-3显示出对2型糖尿病的管理潜力.
科学领域:
- 植物化学 植物化学
- 自然产品化学 自然产品化学
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 帕纳克斯诺托金生叶多糖 (PNLP) 以其潜在的健康益处而闻名.
- 优化提取和净化对于释放它们的治疗性质至关重要,特别是在2型糖尿病等代谢障碍方面.
- 了解PNLP的结构和功能关系是其应用的关键.
研究的目的:
- 使用超声波辅助双酶方法优化从Panax notoginseng叶 (PNLP) 中提取原始多糖.
- 净化和描述活性分数 (PNLP-3) 并评估其低血糖和抗氧化活性.
- 研究PNLP-3在治疗2型糖尿病中的潜力.
主要方法:
- 单因素和响应表面的方法来优化PNLP提取.
- DEAE-纤维素52染色学用于净化PNLP-3.
- 结构分析 (糖成分,分子量),抗氧化剂测试,消化酶抑制测试 (α-葡萄糖酶,α-氨酶),分子对接和基于细胞的测试 (HepG2细胞).
主要成果:
- 经过优化提取,获得了17.13 ± 0.29%的原油PNLP.
- 纯化的PNLP-3 (81.2%总糖,16.57kDa) 显示出强大的α-葡萄糖酶 (IC50 = 1.045 mg/mL) 和α-氨基酶 (IC50 = 9.53 mg/mL) 的抑制.
- 在胰岛素抵抗性HepG2细胞中,PNLP-3增强了葡萄糖消耗和糖原合成,降低了高血糖,并表现出度依赖的抗氧化活性.
结论:
- 优化的超声波辅助双酶方法对PNLP提取有效.
- PNLP-3具有显著的低血糖和抗氧化特性,具有强大的α-葡萄糖酶抑制活性.
- 松树叶多糖,特别是PNLP-3,是开发功能性食品或治疗2型糖尿病管理的治疗剂的有希望的自然资源.
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