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使用下一代测序 (NGS) 在谷物样本中基于rDNA的先进的小麦病原体检测

Katarzyna Pieczul¹, Ilona Świerczyńska¹, Andrzej Wójtowicz¹

¹Institute of Plant Protection-National Research Institute, 60-318 Poznan, Poland.

Pathogens (Basel, Switzerland)

|February 26, 2025

概括

冬季小麦中的核糖体DNA区域的高通量测序揭示了多样化的真菌群落和Fusarium等病原体的严重污染. 这种方法有助于精确的病原体监测,以改善作物健康管理.

科学领域:

植物病理学植物病理学
菌类学菌类学是指菌类学.
基因组学就是基因组学.

背景情况:

传统的植物病理学诊断有其局限性.
高通量测序 (HTS) 提供了精确的病原体监测和识别.
核糖体DNA (rDNA) 区域是真菌分类的可靠标记.

研究的目的:

使用HTS.探索冬季小麦谷物样本中的真菌多样性和病原体存在.
评估ITS1和ITS2区域在真菌分析和病原体检测方面的实用性.

主要方法:

下一代测序 (NGS) 针对核糖体DNA的ITS1和ITS2区域.
分析了249,743个来自ITS1的读数和179,675个来自ITS2.2的读数.
操作分类单位 (OTU) 和真菌分类的识别.

主要成果:

在115种真菌种群中确定了183个OTU.
在ITS1分析中,发现了Fusarium sp. (61%) 占主导地位;ITS2显示Fusarium culmorum (47%) 是最丰富的,表明谷物受到严重污染.
检测到受隔离管制的病原体 (T. caries,T. triticoides) 和其他重要的谷物病原体 (F. cerealis,F. poae,F. tricinctum).

结论:

ITS1 和 ITS2 区域对于在小麦中进行全面的真菌分析和病原体检测是互补的.
针对rDNA区域的HTS为谷物作物中的真菌病原体的监测和管理提供了宝贵的见解.
调查结果强调了改善农业疾病监测和风险评估的潜力.

关键词:

在 ITS1 和 ITS2 系统中.菌是一种真菌.下一代测序 (NGS) 是指下一代的测序.病原体的鉴定病原体的鉴定小麦小麦小麦小麦小麦小麦小麦.

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