适应性实验室进化和碳/不平衡促进了Saccharomyces cerevisiae中的高产氨释放
Alex Pessina1,2, Anna Giancontieri1, Tommaso Sassi1,2
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza Della Scienza 2, 20126 Milan, Italy.
Microorganisms
|February 26, 2025
概括
研究人员通过优化氨基酸代谢来增强酵母中的氨酸产量. 进化酵母菌株分泌的氨量显著增加,证明了哈伯 - 博什工艺对肥料和能源应用的可持续替代品.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 可持续化学 可持续化学
背景情况:
- 氨对于肥料和能源储存至关重要,但主要是通过能源密集的哈伯-博什工艺生产的.
- 微生物氨生产提供了一个可持续的替代方案,但天然酵母菌株的成功程度有限.
- 在酵母中优化氨基酸去胺代谢是提高氨产量的关键.
研究的目的:
- 通过增强氨基酸利用,改善*Saccharomyces cerevisiae*中的氨产量.
- 研究适应性实验室进化的潜力,以增加酵母中的氨分泌.
- 从富含蛋白质的废物中制造酵母式氨的概念验证.
主要方法:
- 应用适应性实验室进化对*Saccharomyces cerevisiae*菌株CEN.PK113-7D使用选择性碳/介质.
- 采用了多步生物反应器培养与缩源用于进化菌株.
- 工程酵母菌株的量化氨生产水平.
主要成果:
- 精选的进化酵母菌株在氨基酸利用和氨分泌中增加了3-5倍.
- 在优化的生物反应器条件下,进化的AAV6菌株产生了1.36g/L的氨.
- 达到与其他已建立的微生物系统可比的氨产量水平.
结论:
- 适应性实验室进化有效地提高了Saccharomyces cerevisiae中的氨产量.
- 基于酵母的氨基合成是一种可行的,可持续的替代传统方法.
- 这项研究为利用高蛋白废物流用于微生物氨生产铺平了道路.
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