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从摩尔多瓦患者中分离出来的抗卡巴尼姆抗性格拉姆阴性细菌的截面评估

Vadim Nirca¹, Frieder Fuchs^2,3,4, Tessa Burgwinkel^4,5

¹Molecular Biology Department, Imunotehnomed Ltd., MD-2001 Chisinau, Moldova.

|February 26, 2025

概括

包括Klebsiella pneumoniae,Acinetobacter baumannii和Pseudomonas aeruginosa在内的耐卡巴尼姆的格拉姆阴性细菌正在摩尔多瓦蔓延. 分子分析显示,与邻国乌克兰共享耐药基因和克隆血统,这表明区域传播.

科学领域:

医学微生物学医学微生物学
分子流行病学分子流行病学
抗微生物耐药性抗微生物耐药性

背景情况:

关于摩尔多瓦格拉姆阴性细菌的分子流行病学和碳烯抗性机制的数据有限.
耐卡巴胺的格拉姆阴性细菌 (CRGNB) 由于治疗选择有限,对全球健康构成重大威胁.

研究的目的:

调查摩尔多瓦格拉姆阴性细菌分离物的分子流行病学和碳烯抗性机制.
在CRGNB分离物中识别流行序列类型和卡巴酶基因.

主要方法:

在11个月内从摩尔多瓦的一家常规诊断实验室收集CRGNB分离物.
表型和基因型抗菌素敏感性测试.
遗传学分析和多位置序列类型 (MLST).

主要成果:

已经发现了克莱布西拉肺炎 (ST101,ST395,ST377),阿西内托巴克特宝曼尼 (ST2,ST19,ST78) 和 Pseudomonas aeruginosa (ST357,ST654) 抗卡巴因菌的集群.
确认了与乌克兰的分离物有遗传学联系,表明了潜在的区域传播.
检测到的碳烯酶基因包括blaOXA-23,blaOXA-72,blaGES-11,blaKPC-3,blaNDM-1,blaOXA-48和blaVIM-2.这些基因都被检测到.

结论:

该研究表明,CRGNB在摩尔多瓦的蔓延,与邻国乌克兰有共同的特征和血统.
可能的溢出事件和由成功的克隆系驱动的区域传播正在促进碳烯耐药性的传播.
隔离物之间的基因组相似性支持了区域传播事件的假设.

关键词:

格拉姆阴性细菌是一种细菌.抗卡巴胺的耐药性流行病学流行病学监控监督监督监督监督监督监督监督监督监督监督监督监督监督监督监督监督监督

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