对口病病毒感染细胞模型系统的比较转录学分析
Haibin Ma1,2, Zhenzhen Zheng2, Min Liu2
1Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricutural University, Guangzhou 510642, China.
Veterinary sciences
|February 26, 2025
概括
在BHK-21细胞中感染口病病毒 (FMDV) 会改变基因表达,影响免疫反应和细胞过程. 已识别的关键枢纽蛋白可能为FMDV控制策略提供目标.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 口疫病毒 (FMDV) 在牲畜中造成重大经济损失.
- 了解宿主对FMDV的反应对于开发有效的治疗方法至关重要.
- BHK-21细胞是FMDV研究的关键模型,因为它们的易感性很高.
研究的目的:
- 为了研究宿主基因表达在BHK-21细胞在FMDV感染后的变化.
- 确定涉及FMDV-BHK-21细胞相互作用的分子通路和关键蛋白质.
- 为开发针对FMDV的新型治疗和诊断策略提供见解.
主要方法:
- 挑战FMDV和控制BHK-21细胞的转录组测序.
- 生物信息分析包括基因本体学 (GO) 和KEGG通路丰富.
- 蛋白质与蛋白质相互作用网络分析以确定枢纽蛋白.
- 选择差异表达基因 (DEGs) 的RT-qPCR验证.
主要成果:
- 鉴定了4018个不同表达的基因,2044个是下调的,1974个是上调的.
- 感染FMDV显著增强了BHK-21细胞的转录启动.
- 缩分析显示了对免疫相关过程和细胞化学物质运输的影响.
- Fos,Flt3lg,Rpl22l1,Ifi35,Ep300和Rps16被确定为关键的枢纽蛋白.
结论:
- 感染FMDV严重改变了BHK-21细胞中的宿主细胞转录组.
- 已识别的枢纽蛋白代表了FMDV治疗干预的潜在目标.
- 这项研究增强了对FMDV病原和宿主病毒相互作用的理解,有助于疾病控制.
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