从反动物中分离酵母对体外酵母发酵的影响
Krung Wilachai1, Pramote Paengkoum1, Nittaya Taethaisong1
1School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Veterinary sciences
|February 26, 2025
概括
从反动物中分离出高性能酵母菌株,以寻找潜在的益生菌发展. 来自奶牛的Dc18菌株表现出优越的耐受性和无氧活力,这使得它对未来的研究充满希望.
科学领域:
- 流体微生物学 流体微生物学
- 动物营养动物营养
- 益生菌酵母的发展
背景情况:
- 食酵母分离物对于开发高性能益生菌菌株至关重要.
- 了解酵母在各种条件下的表现,对于它们在动物料中的应用至关重要.
研究的目的:
- 从反动物来源分离和识别有前途的酵母菌株.
- 根据耐受性,无氧生长和体外发酵特性来评估选定酵母菌株的性能.
- 为了确定一个合适的酵母菌株,以进一步培养益生菌.
主要方法:
- 使用微生物培养,API 20C AUX Kit和26S rRNA基因测序,分离和识别了91种酵母菌株.
- 基于pH耐受度 (3.5-7.5),总挥发性脂肪酸 (TVFA) 耐受度 (0-4%),无氧生长率和体外气体生产的性能评估.
- 在体外发酵试验中,使用乳牛的反液进行测试,以评估对气体动力学和挥发性脂肪酸生产的影响.
主要成果:
- 酵母菌株,包括Candida spp. 和Issatchenkia orientalis,已经确定.
- 菌株Bu3和Dc18在pH和TVFA耐受性测试中表现出卓越的表现.
- 菌株Dc18在无氧条件下增加了可活细胞数量,并在体外增加了黄油酸的产生.
结论:
- 从奶牛中分离出来的Dc18菌株表现出强大的耐受性和无氧活力.
- 已识别的酵母菌株Dc18是作为反动物应用中的益生菌进一步研究的强有力的候选者.
- 酵母培养补充剂在体外发酵中对黄油酸度产生了积极的影响.
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