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基于UHPLC-MS和其应用的Eupolyphaga稳定的指纹概况分析

Xin Lai¹, Hongwei Song¹, Guangli Yan¹

¹State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China.

Pharmaceuticals (Basel, Switzerland)

|February 26, 2025

概括

这项研究开发了一种超高性能液体染色体质谱法 (UHPLC-MS) 方法,用于识别Eupolyphaga steleophaga中的63种酶成分. 这种方法提高了这种传统药物的质量控制,为进一步的药理学研究铺平了道路.

科学领域:

药理学和传统医学的医学.
分析化学分析化学
兽医医学兽医医学兽医医学

背景情况:

Eupolyphaga steleophaga是一种具有已知的健康益处的药物,包括抗瘤和免疫调节性质.
来自Eupolyphaga steleophaga的多具有生物学活性,影响凝血和脂质代谢.
澄清的组成对于质量控制和理解药理效应至关重要.

研究的目的:

在口服后模拟Eupolyphaga steleophaga的消化吸收.
为了识别Eupolyphaga steleophaga的酶成分.
加强对Eupolyphaga steleophaga的质量控制措施.

主要方法:

模拟消化使用人工胃液和pepsin.
开发了一种超高性能液体染色体质谱法 (UHPLC-MS) 指纹采集方法.
使用UHPLC-MS方法分析了10批Eupolyphaga石虫.

主要成果:

在Eupolyphaga steleophaga中成功识别了63个酶成分.
在UHPLC-MS方法证明了高精度,可重复性和稳定性.
在所有分析批量的63个成分中确定了多标记物,验证了质量控制方法.

结论:

新的UHPLC-MS方法是动物制药品质量控制的强大工具.
这种方法为未来对Eupolyphaga steleophaga多的药理学研究提供了可靠的基础.
提高了Eupolyphaga steleophaga生物活动和治疗潜力的探索.

关键词:

欧波利法格斯泰利法格斯多的多.指纹个人资料指纹个人资料质量识别质量识别.超高性能液体染色体质谱仪 (UHPLC-MS) 是一种超高性能液体染色体质谱仪.

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