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电喷式葡萄糖氧化酶囊的封装效率:干燥技术的影响

Minerva Rentería-Ortega¹, María de Jesús Perea-Flores², Alberto Peña-Barrientos²

¹Tecnológico Nacional de México/Tecnológico de Estudios Superiores de San Felipe del Progreso, San Felipe del Progreso 50640, Mexico.

|February 26, 2025

概括

电子喷雾成功封装葡萄糖氧化酶 (GOX) 使用子粘液和酸. 临界点干燥最大限度地提高了封装效率和酶活性,为面包店应用提供了更好的酶递送.

科学领域:

食品科学食品科学食品科学
生物技术是生物技术.
材料科学材料科学材料科学

背景情况:

葡萄糖氧化酶 (GOX) 在面包制品中增强了面团质和面包质量.
直接添加GOX导致过早活动,限制其功能益处.
封装是一种控制酶释放和稳定性的策略.

研究的目的:

用粘液和藻酸盐使用电喷涂来封装葡萄糖氧化酶 (GOX).
评估三种干燥方法 (CPD,LC,OD) 对封装效率 (EE),酶活性和微观结构完整性的影响.
了解封装GOX的释放动力学.

主要方法:

用粘液和藻酸盐对GOX进行电喷.
临界点干燥 (CPD),冷/冷干燥 (LC) 和烤箱干燥 (OD) 的比较.
对封装效率,保留的酶活性和微观结构完整性的分析.

主要成果:

CPD产生了最高的EE (70%),并保留了微囊结构,最大限度地减少了酶泄漏.
由于冰晶的破坏,LC导致中度EE (27.43%).
由于囊缩,OD导致了显著的酶损失 (57.1%).
GOX释放动力学遵循Korsmeyer-Peppas模型,表明非Fickian扩散.

结论:

干燥技术对于封装酶的稳定性和释放行为至关重要.
CPD是保持GOX功能和封装效率的最有效方法.
这项研究为优化食品应用中的酶递送系统提供了洞察力.

关键词:

临界点的干燥临界点的干燥电气喷是一种电子喷.封装的封装方式冰干燥的使用方法葡萄糖氧化酶是什么?烤箱干燥烤箱干燥

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