转录基因组和代谢基因组分析提供了关于黄类生物合成和Zanthoxylum bungeanumMaxim bungeanum中颜色形成机制的见解
Lin Yang1,2, Lu Tian1, Jingwei Shi1,2
1College of Forestry, Northwest A&F University, Yangling 712100, China.
Plants (Basel, Switzerland)
|February 26, 2025
概括
桑托西 (Zanthoxylum bungeanum) 果实的颜色是由tricetin和 (-) -epigallocatechin等黄类化合物决定的. 关键的基因和转录因子调节它们的合成,为作物改进提供了洞察力.
科学领域:
- 植物科学 植物科学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 植物Zanthoxylum bungeanum中的水果颜色是影响工业发展的关键质量特征.
- 尚不清楚Zanthoxylum bungeanum水果颜色形成背后的分子机制.
研究的目的:
- 阐明Zanthoxylum bungeanum中调节色彩形成的分子机制.
- 为了确定关键的代谢物,基因和转录因子参与水果色素化.
主要方法:
- 跨越四个发育阶段的转录组和代谢组概况的综合分析.
- 鉴定差异积累代谢物 (DAMs) 和差异表达基因 (DEGs).
- 对黄类合成基因 (ZbCHI,ZbFLS,ZbANR) 和转录因子 (MYB,bHLH) 的生物信息学分析.
- 通过Nicotiana benthamiana的短暂表达进行功能分析.
主要成果:
- 鉴定出137种黄素,其中三素和 (-) - 甲素与水果颜色密切相关.
- 九个DEG和15个转录因子 (12 MYB,3 bHLH) 被确定为关键调节者.
- ZbCHI,ZbFLS和ZbANR蛋白显示出不同的结构和定位特性.
- 这些基因的短暂表达增加了黄酸含量,ZbANR导致白色表型.
结论:
- 这项研究提供了对黄酸代谢和Zanthoxylum bungeanum颜色形成的分子基础的全面了解.
- 已识别的基因和通路为未来旨在改善水果颜色的育种计划提供了理论基础.
相关概念视频
Monohybrid Crosses
215.1K
Overview
215.1K
Epistasis
37.3K
In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
37.3K
Position-effect Variegation
5.6K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
5.6K
Background and Environment Affect Phenotype
5.8K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
5.8K
Transgenic Plants
7.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.0K
Epistasis Analysis
4.9K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
4.9K


