慢性系统性SARS-CoV-2感染没有呼吸道参与在免疫功能低下的患者
Francisco Tejerina1,2,3, Daniel Peñas-Utrilla1,2, Marta Herranz1,2
1Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, 28007 Madrid, Spain.
在免疫抑制患者的呼吸外样本中,SARS-CoV-2 RNA持续数月. 观察到病毒的演变,表明尽管上呼吸道检测结果呈阴性,但病毒仍然具有生存能力.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 持续的SARS-CoV-2泄露可能发生在免疫功能低下的人群中.
- 呼吸道外的病毒分泌还没有完全被理解.
- 在特定患者群体中,长期病毒活力需要进一步调查.
研究的目的:
- 为了研究SARS-CoV-2在免疫抑制患者中脱落的持续时间和演变.
- 确定在呼吸外部位检测到的病毒RNA是否表明病毒活跃复制和进化.
- 为了评估病毒活力在长时间脱落的背景下.
主要方法:
- 纵向采集呼吸道和呼吸外样本.
- 逆转录定量聚合酶连锁反应 (RT-qPCR) 用于SARS-CoV-2RNA检测.
- 全基因组测序 (WGS) 用于分析随时间推移的病毒遗传变化.
主要成果:
- 在几个月的呼吸外样本中检测到SARS-CoV-2RNA.
- 在此期间,上呼吸道样本对SARS-CoV-2RNA始终为负.
- 全基因组测序揭示了单核酸多态的积累,表明病毒的进化.
结论:
- 这项研究表明,在免疫抑制患者的呼吸外部位,SARS-CoV-2 RNA 脱落的时间长.
- 观察到的病毒进化表明持续的病毒活力和复制.
- 这些发现强调了考虑免疫受损个体的呼吸外流放的重要性.
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