相关实验视频
Updated: May 25, 2025

10:55
Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
19.3K
DDX21通过结合帽子蛋白和抑制干扰素反应促进PCV3复制
Haoyu Sun1,2, Qianhong Dai1,2, Beiyi Zhou1,2
1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Viruses
|February 26, 2025
概括
猪病毒3型 (PCV3) 复制是由DDX21促进的,这是一种与PCV3Cap蛋白结合并抑制干扰素β生产的蛋白质,有助于病毒扩散.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 猪病毒3型 (PCV3) 在猪中引起重大疾病,包括皮肤炎和生殖功能衰竭.
- PCV3囊蛋白 (Cap) 与DDX21相互作用,DDX21是一种参与干扰素-β调节的蛋白质.
- 在此之前,DDX21-PCV3 Cap相互作用在病毒复制中的确切作用是未知的.
研究的目的:
- 阐明DDX21影响PCV3复制的机制.
- 调查PCV3 Cap和DDX21之间的相互作用及其功能后果.
- 确定DDX21在PCV3感染期间宿主的干扰素反应中的作用.
主要方法:
- 在PK-15细胞中使用shRNA消耗DDX21.
- 通过病毒标位和复制酶 (Rep) 蛋白水平评估PCV3扩散.
- 对干扰素-β (IFN-β) 和干扰素刺激基因表达 (ISG) 的分析.
- 使用PCV3 Cap和DDX21的同局部化和相互作用研究,包括对特定域的分析.
主要成果:
- 减少DDX21显著影响PCV3增殖,减少病毒标位和Rep蛋白水平.
- 发现DDX21可以负面调节IFN-β和ISG水平,从而促进PCV3复制.
- PCV3 Cap和DDX21之间的相互作用需要Cap的核定位信号 (NLS) 和DDX21的特定C端域区域.
- 观察到PCV3感染会干扰DDX21的抑制功能,增强其亲病毒活性.
结论:
- DDX21通过与PCV3 Cap蛋白相互作用来促进PCV3复制.
- 这种相互作用抑制了宿主的干扰素-β反应,为病毒增殖创造了有利的环境.
- 了解这种相互作用为开发预防和控制PCV3感染的策略提供了洞察力.
相关概念视频
Immune Response Against Viral Pathogens
728
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
728
Retrovirus Life Cycles
45.6K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.6K
Eukaryotic Transcription Inhibitors
9.8K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.8K
Retroviruses
12.2K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.2K
Inhibition of Cdk Activity
4.6K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.6K
Cytotoxic T Cells-mediated Immune Response
826
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
826

