在Cytospora chrysosperma中识别Mycoviruses:对于核桃瘤的潜在生物控制剂
Yingjie Mi1, Shaohua Chen2, Kexin Liu1
1Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, College of Agriculture, Shihezi University, Shihezi 832003, China.
Viruses
|February 26, 2025
概括
在核桃癌菌Cytospora chrysosperma中发现了七种新的真菌病毒. 一些真菌病毒显著降低了真菌的毒性,为这种植物疾病提供了潜在的生物控制.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 病毒学 病毒学
背景情况:
- 核桃,由Cytospora chrysosperma引起,是中国新疆地区的一个重要疾病,没有有效的控制.
- 已知mycoviruses可以降低病原菌的毒性,并且可以作为生物控制剂.
研究的目的:
- 为了识别与新疆的Cytospora chrysosperma菌株相关的真菌病毒.
- 为了研究这些真菌病毒作为生物控制剂对抗核桃癌的潜力.
主要方法:
- 超转录组测序被用来识别31种C. chrysosperma菌株中的真菌病毒.
- 用BLAST和RT-PCR分析来识别和确认真菌病毒.
- 进行了共同培养实验,以评估mycoviruses对真菌生长和毒性的影响.
主要成果:
- 发现了七种新型的真菌病毒,分别是Botrytis cinerea partitivirus 5 (BcPV5),Gammapartitivirus sp-XJ1 (GVsp-XJ1),Botoulivirus sp-XJ2 (BVsp-XJ2),Luoyang Fusar虫病毒 2 (LfTV2),Leptosphaeria biglobosa narnavirus 17 (LbNV17),Sclerotinia sclerotiorum narnavirus 6 (SsNV6),以及Cytospora ribis mitovirus (Cr3).MV3) 这三种新型的真菌病毒都被发现.
- 其中五种真菌病毒 (BcPV5,GVsp-XJ1,BVsp-XJ2,CrMV3和LfTV2) 被发现同时感染C. chrysosperma菌株WS-11.
- 同时感染显著降低了殖民地生长率和C. chrysosperma的毒性. 没有病毒的菌株,当被感染时,也显示出增长和毒性下降.
结论:
- 这项研究发现了七种与C. chrysosperma相关的新型真菌病毒.
- 几种已识别的真菌病毒显示,宿主真菌的毒性显著降低.
- 这些真菌病毒代表了有希望的生物控制资源,用于管理核桃癌症.
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