由饮食-脂质刺激调节的肠道IncRNAs和circRNAs
Luis A Chapado1, Carmen Crespo2, Joao Tomé-Carneiro2
1Laboratory of Epigenetics of Lipid Metabolism, Madrid Institute for Advanced Studies Food (IMDEA Food), CEI UAM + CSIC. Carretera de Cantoblanco, 8. 28049., Madrid, Spain.
Molecular nutrition & food research
|February 26, 2025
概括
高脂肪饮食会改变肠道中非编码RNA的表达. 研究人员在小鼠和人类器官中发现了长非编码RNA (lncRNA) 和圆形RNA (circRNA) 的显著变化,这表明它们在脂质代谢中起作用.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 高脂肪饮食与各种健康问题有关.
- 非编码RNAs (ncRNAs),包括长非编码RNAs (lncRNAs) 和圆形RNAs (circRNAs),是关键的调节分子.
- 食脂质对肠道ncRNA表达的影响尚未完全理解.
研究的目的:
- 为了研究高食脂类暴露对肠道lncRNA和circRNA表达的影响.
- 为了比较ncRNA表达的变化 in vivo (小鼠) 和 in vitro (人类肠道器官).
主要方法:
- 在急性脂质挑战和慢性高脂肪饮食后,对小鼠的lncRNAs和circRNAs的查.
- 在脂质暴露后对人类肠道有机体中的lncRNA和circRNA表达的分析.
- 在体内和体外模型之间差异表达的ncRNAs的比较分析.
主要成果:
- 在体内,一种脂质挑战在3小时内调节了15个lncRNA和41个circRNA.
- 慢性高脂肪饮食在小鼠中4天后改变了11个 lncRNAs.
- 在体外,251个上调和387个下调的lncRNA和19个上调和16个下调的circRNA在暴露后3小时内被确定.
- 在小鼠和人类器官之间的ncRNA反应中观察到显著的特定物种差异.
结论:
- 饮食中的高脂类摄入量显著改变了肠道中lncRNAs和circRNAs的表达.
- 这些发现表明ncRNAs在脂质代谢调节中的潜在作用.
- 在小鼠和人类器官之间的ncRNA表达模式中存在有限的一致性,突出了特定物种的反应.
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