辐射诱导对人类乳腺癌细胞的救援效应是由巨细胞调节的
Spoorthy Pathikonda1, Li Tian2, Clement Manohar Arava3
1Departments of Chemistry, City University of Hong Kong, Kowloon Tong, Hong Kong Special Administrative Region of China.
Biochemistry and biophysics reports
|February 26, 2025
概括
辐射诱导救援效应 (RIRE) 保护癌细胞免受DNA损伤. 巨细胞两极分化显著影响RIRE,通过调节瘤微环境中的保护信号,影响放射治疗的有效性.
科学领域:
- 癌症生物学 癌症生物学
- 放射治疗研究 放射治疗研究
- 免疫学 免疫学 免疫学
背景情况:
- 瘤微环境影响癌细胞对DNA损伤的易感性.
- 辐射诱导救援效应 (RIRE) 描述了邻近细胞如何减少被辐射细胞中的DNA损伤.
- 了解RIRE对于优化放射治疗疗效至关重要.
研究的目的:
- 研究和描述不同类型的RIRE.
- 在RIRE中探索旁观者细胞类型和极化状态的作用.
- 确定巨分极对RIRE的影响及其对放射治疗的影响.
主要方法:
- 癌细胞 (MCF7) 与旁观者细胞 (天真或预先条件) 的共同培养.
- 使用-H2AX和53BP1免疫染色对紫外线诱导的DNA损伤的评估.
- 在不同巨细胞极化状态 (M1,M2,TAM) 中对RIRE的调制和评估.
主要成果:
- 确定了两种类型的RIRE:基底RIRE和活性RIRE.
- MCF7细胞表现出显著的活性RIRE,而THP1衍生的巨细胞显示出强大的基底RIRE.
- 巨细胞极化对RIRE进行了批判性调节:M1抑制了它,而M2和TAM增强了它.
结论:
- 天生的免疫系统,特别是巨细胞,在RIRE中发挥着重要作用.
- 巨细胞极化状态决定了它们影响RIRE的能力,影响放射治疗结果.
- 与瘤相关的巨细胞和M2巨细胞增强RIRE,可能降低放射治疗的有效性.
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