对Limosilactobacillus fermentum SD7的亲生基因组分析,这是一个具有显著聚合能力的益生菌候选者
Thunchanok Yaikhan1, Monwadee Wonglapsuwan2, Nuntiya Pahumunto3,4
1Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Heliyon
|February 26, 2025
概括
从人类口腔中分离出来的Limosilactobacillus fermentum SD7,显示出作为益生菌的前景. 它的基因组分析揭示了对益生菌有效性至关重要的聚合基因,没有检测到有害的耐药性或毒性基因.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 益生菌 益生菌 益生菌
背景情况:
- Limosilactobacillus fermentum以其免疫调节作用而闻名.
- 益生菌是有益的微生物,用于食品和医疗保健.
- 人口腔是各种细菌物种的息地,具有潜在的益生菌价值.
研究的目的:
- 为了描述从人类口腔中分离出来的Limosilactobacillus fermentum SD7的基因组.
- 用基因组数据将L. fermentum SD7与其他L. fermentum菌株进行比较.
- 确定有助于L. fermentum SD7.7益生菌潜力的遗传因素.
主要方法:
- 全基因组测序和分析L. 发酵 SD7.
- 涉及其他153个L. fermentum基因组的比较基因组学.
- 使用核心基因单核酸多态性基因的植物遗传学分析.
- 核心和辅助基因的识别,包括与CRISPR相关的基因,抗菌素耐药性和毒性基因.
- 亲生基因组分析以评估聚合能力.
主要成果:
- L. fermentum SD7 的基因组是 2.27 Mb 的,含有 51.1% 的 GC.
- 遗传学分析将154种L. fermentum菌株分为6个分类,显示L. fermentum SD7与FS-10和L13菌株相似.
- L. fermentum SD7拥有910个核心基因和10,169个辅助基因,包括四个独特的CRISPR相关基因.
- 在L. fermentum SD7基因组中没有发现抗微生物耐药性或毒性基因.
- 确定了与聚合能力相关的27个基因,支持其益生菌潜力.
结论:
- 由于其遗传构成,Limosilactobacillus fermentum SD7 是一个有前途的益生菌候选人.
- 已识别的聚合基因对其益生菌疗效至关重要.
- L. fermentum SD7缺乏与抗菌素耐药性和毒性相关的基因,从而提高了其安全性.
- 这项研究为潜在的食品和医疗保健应用提供了对L. fermentum SD7的全面基因组理解.
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