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Vaccine Production

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

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Updated: May 12, 2026

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

这是一个新的平台,用于设计基于AAV的疫苗.

Sabrina Babutzka¹, Miranda Gehrke¹, Anastasia Papadopoulou²

¹Department of Ophthalmology, University Hospital, LMU Munich, 80336 Munich, Germany.

Molecular therapy. Methods & clinical development

|February 26, 2025

概括

工程设计的腺相关病毒 (AAV) 囊体显示SARS-CoV-2抗原,创造了一个新的疫苗平台. 这个平台引发了强大的幽默和细胞免疫反应对抗病毒.

关键词:

在 AAV AAV AAV 中.卡普西德 (Capsid) 是一种体.细胞反应的细胞反应.工程化囊体工程化囊体幽默反应是一种幽默反应.蛋白质载体蛋白质载体疫苗疫苗疫苗疫苗类似病毒的颗粒.

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Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

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Published on: January 29, 2019

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

相关实验视频

Last Updated: May 12, 2026

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

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Published on: January 29, 2019

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

科学领域:

病毒学病毒学
免疫学免疫学免疫学
生物技术是生物技术.

背景情况:

基因相关病毒 (AAV) 体工程增强了基因治疗载体.
AAV技术可以适应基因疗法以外的应用,例如疫苗开发.

研究的目的:

为了研究改造的AAV体作为展示大型免疫原性抗原的支架.
开发一种使用AAV的新型疫苗平台,以引起针对SARS-CoV-2等病原体的强烈,特定的免疫反应.

主要方法:

通过引入大约200个SARS-CoV-2受体结合域 (RBD) 的氨基酸,改造了AAV2和AAV9囊.
创建了AAV2.RBD和AAV9.RBD囊体 (AAV.RBDs),并评估了它们的SARS-CoV-2类属性,包括血管酶转化酶2受体亲和力.
测试了接种疫苗的捐赠者的人体血清的中和,并评估了子和人类淋巴细胞的免疫反应.

主要成果:

工程AAV.RBDs表现出类似SARS-CoV-2的特性,包括ACE2受体结合.
通过针对SARS-CoV-2接种疫苗的个体的血清中和了AAV.RBD.
在子体内进行皮下注射,诱导了针对SARS-CoV-2 RBD的强烈幽默反应,并在人类淋巴细胞中触发了RBD特异性的细胞免疫力.

结论:

开发了一个基于AAV的新型疫苗平台,用于呈现大型抗原序列.
这个平台引发了强烈和特定的免疫反应,为具有挑战性免疫策略的疾病提供了多功能方法.