基因多态化和蛋白质表达与甲状腺结结节患者的组织病变变的关联 VDR/Foki
Mariwan F Abdalfatah1,2, Abdullah A Shareef3, Lana S Saleh3
1Medical Laboratory Science Department, Halabja Technical College, Sulaimani Polytechnic University, Sulaymaniyah, Kurdistan Region, Iraq.
Analytical cellular pathology (Amsterdam)
|February 26, 2025
概括
维生素D受体 (VDR) 表达和VDR/FokI基因型影响着结结的发展. TT基因型提供了保护,而VDR表达与结节的大小和数量相关.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 状结节是常见的良性甲状腺病变,通常进展缓慢.
- 虽然通常无症状,但未经治疗的结结节可能会带来恶性转变的风险.
- 了解影响体结节敏感性的因素对于临床管理至关重要.
研究的目的:
- 为了研究维生素D受体 (VDR) 在甲状腺结体中的表达作用.
- 分析VDR/FokI (rs2228570) 基因型与体结节敏感性之间的关联.
- 为了确定血清维生素D水平与体结节的发展之间的相关性.
主要方法:
- 用PCR确定VDR FokI基因型,用于甲固定嵌 (FFPE) 块和血液样本.
- 在甲状腺组织部分,通过免疫组织化学评估VDR蛋白表达.
- 血清维生素D水平使用酶相关免疫吸收试验 (ELISA) 进行测量.
主要成果:
- 在77.5%的病例中观察到强烈的细胞质VDR表达,与较大的结节大小和多结节状况相关.
- 对VDR/FokI的TT同卵性基因型被确定为对结节的显著保护因素 (OR:0.11).
- 结节大小在CC基因型携带者中显著更大,血清维生素D水平在患者和对照人群之间没有显著差异.
结论:
- 在体结节中突出的细胞质VDR表达表明了特定的分布模式和潜在的作用.
- 随着结节的大小和数量增加,VDR表达强度会增加,这表明可能与疾病进展有关.
- TT基因型似乎对合结形成有保护作用,为未来的研究和临床策略提供了洞察力.
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