GLASSR-Net:玻璃基板光谱恢复神经网络用于指纹区域的里埃变换红外显微镜.
Xiangyu Zhao1, Jingzhu Shao1, Yudong Tian1
1Center for Biophotonics, Institute of Medical Robotics, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Analytical chemistry
|February 26, 2025
概括
福利埃变换红外光谱 (FTIR) 显微镜现在可以使用具有成本效益的玻璃幻灯片. 一个新的神经网络,GLASSR-Net,恢复了光谱数据,使这种病理学工具的临床应用更广泛.
科学领域:
- 生物医学工程 生物医学工程
- 频谱学是一种光谱学.
- 计算病理学计算病理学
背景情况:
- 福里埃变换红外光谱 (FTIR) 显微镜是疾病生物标志物识别的宝贵工具.
- 目前的局限性包括依赖昂贵和脆弱的红外透明基板 (例如,CaF2/BaF2) 或不透明的红外反射基板.
- 病理玻璃幻灯片在临床环境中具有成本效益和标准,但对于FTIR显微光谱来说并不理想.
研究的目的:
- 开发和验证一种方法,直接从标准玻璃病理基板收集高质量的FTIR光谱.
- 建立一种计算方法来恢复在使用玻璃幻灯片时丢失的光谱信息.
- 为了使FTIR显微光谱能够整合到常规的临床组织学工作流程中.
主要方法:
- 发展玻璃基板光谱恢复神经网络 (GLASSR-Net).
- 在玻璃和CaF2基板上采集 FTIR 光扫描数据,用于状甲状腺癌 (PTC) 的连续组织部分.
- 培训和验证GLASSR-Net使用基于玻璃的光谱作为输入和基于CaF2的光谱作为地面真相.
主要成果:
- GLASSR-Net成功地从基于玻璃的FTIR数据中恢复了指纹吸收光谱 (1800-1000 cm-1).
- 恢复的光谱准确地重建了空间和光谱领域的生物化学分布.
- 对恢复光谱的分析揭示了PTC特有的生化特征,包括胺I/II吸收减少和癌症区域脂质/核酸增加.
结论:
- GLASSR-Net为玻璃基FTIR显微光谱中的光谱恢复提供了一个新的框架.
- 这种方法克服了基质的局限性,促进了FTIR显微光谱学的临床转化.
- 该方法将传统的光谱组织学与先进的计算方法相结合,用于增强病理分析.
相关概念视频
Infrared (IR) Spectroscopy: Overview
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
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When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
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IR Spectrometers
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
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