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确定miR-34c-5p作为与黑山羊丸发育相关的潜在调节分子

Yunchuan Zhang¹, Peng Qiu¹, Junzhi Lu¹

¹Guangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

|February 26, 2025

概括

微RNAs (miRNAs) 调节黑山羊丸的发育. 在成熟的丸中高度表达的miR-34c-5p抑制了塞尔托利细胞的增殖,并影响了生长因子,这表明它在青春期和繁殖中的作用.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
遗传学是一个遗传学.

背景情况:

塞尔托利细胞 (SC) 的生长和成熟对丸发育至关重要.
微RNAs (miRNAs) 正在成为这一过程中的关键调节者.
了解miRNA参与可以为改善畜牧养殖的策略提供信息.

研究的目的:

系统地选和分析黑山羊丸发育中的关键miRNAs.
确定参与精子生成的候选信使RNA (mRNA) 的潜在机制.
为了研究miR-34c-5p在塞尔托利细胞中的功能作用.

主要方法:

来自不同发育阶段的黑山羊丸的miRNA转录资料的构建和比较分析 (3, 30, 90 和 180 天).
差异表达的miRNA基因的生物信息预测和丰富分析.
在体外实验涉及miR-34c-5p过度表达和RNA干扰的Sertoli细胞.

主要成果:

在30天和90天的黑山羊丸之间观察到miRNA表达的最显著差异.
预测的目标基因在细胞增殖,组成和信号通路上得到了丰富,如p53,FoxO和PI3K-Akt.
miR-34c-5p在90天的丸中高度表达,向IGF1R并抑制SC增殖,增加细胞亡,并减少生长因子表达 (CXCL12,BMP4,GDNF,IGF1).

结论:

miR-34c-5p在调节黑山羊丸发育方面发挥着重要作用.
这种miRNA可以抑制塞尔托利细胞的增殖,并调节关键生长因子.
miR-34c-5p是调节丸发育的潜在目标,也是黑山羊育种计划中青春期的标志物.

关键词:

miR-34c-5p 的时间.塞尔托利细胞是Sertoli细胞.黑山羊是一只黑山羊.精子的产生是精子的产生.丸的发展丸的发展

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