标识和验证常见小麦 (Triticum aestivum L.) 七个与质量相关的特征的定量特征位置
Zhi Li1,2,3, Xinli Li2,3, Sunhong Liu2
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China.
概括
研究人员为小麦谷物质量绘制了六个主要和稳定的定量特征位点 (QTL),确定了改善小麦育种的新遗传资源. 这些QTL控制蛋白质和粉含量等特征,这对于高质量的小麦生产至关重要.
科学领域:
- 植物遗传学 植物遗传学
- 农业科学 农业科学
- 分子育种是一种分子育种.
背景情况:
- 小麦谷物质量对经济价值至关重要,受遗传和环境因素的重大影响.
- 了解这些特征的遗传基础对于开发改进的小麦品种至关重要.
研究的目的:
- 为七个主要的小麦谷物质量特征绘制定量特征位置 (QTL).
- 为未来的小麦育种应用确定主要和稳定的QTL.
主要方法:
- 在304个重组杂交系的种群中,使用Wheat55K SNP Array中的8329个标记构建了一个遗传链接地图.
- 量化特征位点 (QTLs) 用ICIM方法绘制了质素的胀指数,SDS沉积量 (SDSS),湿质含量,谷物蛋白含量,质指数,谷物粉含量和下降数量的地图.
- 竞争性等位基特异性PCR (KASP) 标记被设计用于已识别的主要QTLs.
主要成果:
- 总共有92个QTLs,194个条件QTLs (cQTLs) 和117对表达QTLs (eQTLs) 被映射出来.
- 已经确定了六个主要和稳定的QTL (QGPC.sau-4A.1,QWGC.sau-4A,QSDSS.sau-1A.1,QGI.sau-1A,QFN.sau-4D,QSIG.sau-1A),其中五个是新的.
- 检测到8个含有19个QTL的QTL集群,包含所有已识别的主要和稳定的QTL.
结论:
- 六个主要和稳定的QTLs的遗传效应在不同人群中得到了验证.
- 这些已识别的QTL和开发的KASP标记器代表了在培育高质量的小麦品种中使用标记器辅助选择的宝贵资源.
- 这项研究为未来研究小麦谷物质量特征的遗传结构的研究提供了基础.
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